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dc.contributor.authorIrino, Kinue
dc.contributor.authorKato, Maria Aide M. F.
dc.contributor.authorVaz, Tania Mara Ibelli
dc.contributor.authorRamos, Isabel Irino
dc.contributor.authorSouza, Maria de Fátima Costa de
dc.contributor.authorCruz, A. S.
dc.contributor.authorGomes, Tania Aparecida Tardelli [UNIFESP]
dc.contributor.authorVieira, Monica Aparecida Midolli [UNIFESP]
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.identifier.citationVeterinary Microbiology. Amsterdam: Elsevier B.V., v. 105, n. 1, p. 29-36, 2005.
dc.description.abstractIn order to determine the occurrence, serotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) strains, 153 fecal samples of cattle randomly selected from six dairy farms in São Paulo State. Brazil. were examined for Shiga toxin (Stx) production by the Vero cell assay. Feces were directly streaked onto MacConkey Sorbitol Agar and incubated at 37degreesC overnight. Sorbitol-negative colonies (maximum 20) and up to 10 sorbitol-positive colonies from each plate were subcultured onto presumptive diagnostic medium IAL. Sorbitol-negative isolates were screened with O157 antiserum for identification of O157:H7 E. coli. Isolates presenting cytotoxic activity were submitted to colony hybridization assays with specific DNA probes for stx1, stx2, eae, Ehly and astA genes. the isolation rate of STEC ranged from 3.8 to 84.6% depending on the farm analysed. STEC was identified in 25.5% of the animals, and most of them (64.1%) carried a single STEC serotype. A total of 202 STEC isolates were recovered from the animals. and except for the 2 O157:H7 isolates all the others expressed cytotoxic activity. the great majority of the STEC isolates carried both stx1 and stx2 genes (114/202, 56-4%) or stx2 (82/202, 40.6%); and whereas the Ehly sequence occurred in most of them (88%) eae was only observed in O151:H7 and O111:HNM isolates. Serotypes O113:H21, O178:H19 and O79:H14 were the most frequent STEC serotypes identified and widely distributed among animals from different farms, while others such as O77:H18. O88:H25 and O98:H17 occurred only in particular farms. This is the first report on the occurrence of STEC in dairy cattle in São Paulo State. and the results point to substantial differences in rate of isolation. serotypes and genetic profile of STEC that has been previously described among beef cattle in our community. Moreover, to our knowledge O79:H14 and O98:H17 represent new STEC serotypes, while O178:H19 has only been recently reported in Spain. (C) 2004 Elsevier B.V. All rights reserved.en
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Microbiology
dc.rightsAcesso restrito
dc.subjectEscherichia coli O157en
dc.subjectShiga toxin-producing E. colien
dc.subjectDairy cattleen
dc.titleSerotypes and virulence markers of Shiga toxin-producing Escherichia coli (STEC) isolated from dairy cattle in São Paulo State, Brazilen
dc.contributor.institutionInst Adolfo Lutz Registro
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationInst Adolfo Lutz Registro, BR-01246902 São Paulo, Brazil
dc.description.affiliationInst Biol, BR-04014002 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science

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