A proteinase inhibitor from Caesalpinia echinata (pau-brasil) seeds for plasma kallikrein, plasmin and factor XIIa

A proteinase inhibitor from Caesalpinia echinata (pau-brasil) seeds for plasma kallikrein, plasmin and factor XIIa

Author Cruz-Silva, Ilana Autor UNIFESP Google Scholar
Gozzo, Andrezza Justino Autor UNIFESP Google Scholar
Nunes, Viviane A. Autor UNIFESP Google Scholar
Carmona, Adriana K. Autor UNIFESP Google Scholar
Faljoni-Alario, Adelaide Google Scholar
Oliva, Maria Luiza V Google Scholar
Sampaio, Misako U. Autor UNIFESP Google Scholar
Sampaio, Claudio A M Autor UNIFESP Google Scholar
Araujo, Mariana S. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Caesalpinia echinata is a tree belonging to the Leguminosae family. the red color of the trunk, looking like burning wood ('brasa' in Portuguese), is the origin of the name Brazil. Seeds of leguminous plants contain high amounts of serine proteinase inhibitors that can affect different biological processes. Here we show that a protein isolated from seeds of C. echinata is able to inhibit enzymes that participate in blood coagulation and fibrinolysis. This inhibitor (CeKI) was purified to homogeneity by ion exchange and reversed-phase chromatography. SDS-PAGE indicated a single polypeptide chain with a molecular mass of 20 kDa. CeKI inhibits human plasma kallikrein K-i=3.1 nm), plasmin K-i=0.18 nm), factor XIIa (K-i=0.18 nm), trypsin K-i=21.5 nm) and factor Xa K-i=0.49 mm). CeKI inhibited kinin release from high-molecular-mass kininogen by kallikrein in vitro. the N-terminal sequence, determined by automatic Edman degradation, identified the inhibitor as a member of the Kunitz family. the secondary structure, determined by circular dichroism, is mainly a random coil followed by P-sheet structure. the action of CeKI on enzymes of the blood-clotting intrinsic pathway was confirmed by prolongation of the activated partial thromboplastin time.
Keywords amino acid sequence
Caesalpinia echinata
circular dichroism
kinin release
Kunitz-type inhibitor
serine proteinase inhibitor
Language English
Date 2004-11-01
Published in Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 385, n. 11, p. 1083-1086, 2004.
ISSN 1431-6730 (Sherpa/Romeo, impact factor)
Publisher Walter de Gruyter & Co
Extent 1083-1086
Origin http://dx.doi.org/10.1515/BC.2004.140
Access rights Closed access
Type Article
Web of Science ID WOS:000225438200013
URI http://repositorio.unifesp.br/handle/11600/28018

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