Boophilus microplus tick larvae, a rich source of Kunitz type serine proteinase inhibitors

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dc.contributor.author Sasaki, S. D.
dc.contributor.author Azzolini, SSA
dc.contributor.author Hirata, I. Y.
dc.contributor.author Andreotti, R.
dc.contributor.author Tanaka, A. S.
dc.date.accessioned 2016-01-24T12:37:20Z
dc.date.available 2016-01-24T12:37:20Z
dc.date.issued 2004-09-01
dc.identifier http://dx.doi.org/10.1016/j.biochi.2004.09.010
dc.identifier.citation Biochimie. Paris: Editions Scientifiques Medicales Elsevier, v. 86, n. 9-10, p. 643-649, 2004.
dc.identifier.issn 0300-9084
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27910
dc.description.abstract Serine proteinase inhibitors from Boophilus microplus tick larvae (BmTIs) were purified by affinity chromatography on a trypsin-Sepharose column. BmTIs presented molecular weight between M, 6200 and 18,400 and inhibitory activity for trypsin, HuPK (human plasma kallikrein) and neutrophil elastase. Using ion exchange chromatography, BmTIs were separated in several protein pools named BmTI-A to BmTI-F and BmTI-1 to BmTI-7. All BmTI forms presented inhibitory activity for trypsin with apparent dissociation constants (K-i) in the nM range. in this work, we describe the purification of BmTI-D, BmTI-2, and BmTI-3. These three inhibitors affected neutrophil elastase and HuPK with K-i also in nM range. BMTI-D proved to be the best HuPK inhibitor, while BmTI-3 was more efficient for neutrophil elastase with dissociation constants (K-i) of 12 and 0.5 nM, respectively. BmTI-D. BmTI-2, and BmTI-3 N-terminal amino acid sequences allowed us to include them into the BPTI-Kunitz type serine proteinase inhibitor family. BmTIs purified on trypsin-Sepharose were also used in a bovine immunization assay, resulting in antibody (anti-BmTIs) production. (C) 2004 Elsevier SAS. All rights reserved. en
dc.format.extent 643-649
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Biochimie
dc.rights Acesso restrito
dc.subject serine proteinase inhibitors en
dc.subject tick en
dc.subject ectoparasite en
dc.subject Boophilus microplus en
dc.title Boophilus microplus tick larvae, a rich source of Kunitz type serine proteinase inhibitors en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.description.affiliation Universidade Federal de São Paulo, Dept Biofis, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliation EMBRAPA, CNPGC, Campo Grande, MS, Brazil
dc.description.affiliation UNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biofis, Escola Paulista Med, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/j.biochi.2004.09.010
dc.description.source Web of Science
dc.identifier.wos WOS:000225621600007



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