Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase

Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase

Author Stumpp, Taiza Autor UNIFESP Google Scholar
Sasso-Cerri, Estela Autor UNIFESP Google Scholar
Freymüller-Haapalainen, Edna Autor UNIFESP Google Scholar
Miraglia, Sandra Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Etoposide is a podophyllotoxin semiderivative that is used in a variety of chemotherapy treatments, including therapy for children tumors. This drug promotes the formation of a ternary DNA-topoisomerase II-etoposide complex that triggers apoptosis. the purpose of this work was to analyze the occurrence of apoptosis in the seminiferous epithelium of prepubertal, pubertal, and adult rats treated with 10, 20, and 40 mg/Kg of etoposide during the prepubertal phase, as well as the role of apoptosis in etoposide-induced testicular damage. the rat testes were fixed in Bouin's liquid, and the apoptotic cells were quantified by means of the hematoxylin and eosin (H&E) technique (all groups) and the terminal dUTP nick end labeling (TUNEL) method (prepubertal groups only). the results obtained from both the H&E and TUNEL methods showed an increased frequency of apoptosis in the seminiferous epithelium of treated animals, except for the subgroup that received the 10-mg/Kg dose and was sacrificed 12 hr after the treatment and for the etoposide-treated pubertal group, that did not show cells suggesting apoptosis during H&E analysis. the labeled cells were mainly primary spermatocytes and differentiated spermatogonia. the prepubertal rats showed an etoposide-dose-dependent diminution of differentiated spermatogonia. Etoposide treatment during the prepubertal phase increases the frequency of apoptosis in the seminiferous epithelium, and causes serious harm to male fertility. (C) 2004 Wiley-Liss, Inc.
Keywords testis
etoposide
apoptosis
rat
spermatogenesis
Language English
Date 2004-07-01
Published in Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 279A, n. 1, p. 611-622, 2004.
ISSN 0003-276X (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 611-622
Origin http://dx.doi.org/10.1002/ar.a.20045
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000222466000005
URI http://repositorio.unifesp.br/handle/11600/27797

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