Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method

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dc.contributor.author Endo, S.
dc.contributor.author Komori, T.
dc.contributor.author Ricci, Giannina [UNIFESP]
dc.contributor.author Sano, A.
dc.contributor.author Yokoyama, K.
dc.contributor.author Ohori, A.
dc.contributor.author Kamei, K.
dc.contributor.author Franco, Marcello Fabiano de [UNIFESP]
dc.contributor.author Miyaji, M.
dc.contributor.author Nishimura, K.
dc.date.accessioned 2016-01-24T12:37:07Z
dc.date.available 2016-01-24T12:37:07Z
dc.date.issued 2004-05-01
dc.identifier http://dx.doi.org/10.1016/j.femsle.2004.03.015
dc.identifier.citation Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.
dc.identifier.issn 0378-1097
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27715
dc.description.abstract Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. en
dc.format.extent 93-97
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Fems Microbiology Letters
dc.rights Acesso aberto
dc.subject gp43 gene en
dc.subject Paracoccidioides brasiliensis en
dc.subject paracoccidioidomycosis en
dc.title Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Chiba Univ
dc.contributor.institution Fujisawa Pharmaceut Co Ltd
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Chiba Univ, Pathogen Fungi & Microbial Toxicoses Res Ctr, Chuo Ku, Chiba 2608673, Japan
dc.description.affiliation Fujisawa Pharmaceut Co Ltd, Analyt Res Labs, Osaka, Japan
dc.description.affiliation Universidade Federal de São Paulo, Fac Med, Dept Anat & Patol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Fac Med, Dept Anat & Patol, São Paulo, Brazil
dc.identifier.doi 10.1016/j.femsle.2004.03.015
dc.description.source Web of Science
dc.identifier.wos WOS:000221218300013



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