Ocular toxoplasmosis: the role of retinal pigment epithelium migration in infection

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dc.contributor.author Tedesco, R. C.
dc.contributor.author Smith, R. L.
dc.contributor.author Corte-Real, S.
dc.contributor.author Calabrese, K. S.
dc.date.accessioned 2016-01-24T12:37:05Z
dc.date.available 2016-01-24T12:37:05Z
dc.date.issued 2004-04-01
dc.identifier http://dx.doi.org/10.1007/s00436-003-1031-2
dc.identifier.citation Parasitology Research. New York: Springer-verlag, v. 92, n. 6, p. 467-472, 2004.
dc.identifier.issn 0932-0113
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27691
dc.description.abstract Our aim was to study the migration of retinal pigmented epithelium (RPE) into the retinal layer during infection of C57BL/6 mice with Toxoplasma gondii. Eyes from infected and non-infected animals were analyzed on the 60th day of infection by light and transmission electron microscopy. Non-infected eyes showed a normal morphology. in contrast, we observed free parasites in the retinal vasculature, the presence of mononuclear inflammatory infiltrate (MNII) and parasites in the vasculature of choroids in infected eyes. No inflammatory infiltrate was observed; RPE cells were identified near the MNII in nuclear and plexiforme layers. RPE cells were also found on the ganglion cell layer and in the outer segments of the photoreceptor. the morphology showed that RPE cells caused a discontinuity in the nuclear and plexiforme layers. Clusters of parasites were found surrounded by RPE cells and MNII in the inner plexiforme layers. Ultrastructural analysis showed that RPE cells migrated through the epithelium into the inner retinal layers. We did not observe Toxoplasma cysts in many eyes in which pathological changes were detected. Only 8.3% of the animals had Toxoplasma cysts in the inner nuclear layer in the absence of inflammatory cells. the migration of RPE cells can be triggered by a disruption of the RPE monolayer or injury to the neural retina, as in the case of toxoplasmosis. en
dc.format.extent 467-472
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof Parasitology Research
dc.rights Acesso restrito
dc.title Ocular toxoplasmosis: the role of retinal pigment epithelium migration in infection en
dc.type Artigo
dc.rights.license http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institution Fundacao Oswaldo Cruz
dc.contributor.institution Fiocruz MS
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Pavilhao Carlos Chagas, Dept Ultraestrutura & Biol Celular,Lab Ultraestru, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliation Fiocruz MS, IOC, Dept Protozool, Lab Imunomodulacao, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Disciplina Anat Descrit & Topog, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Disciplina Anat Descrit & Topog, São Paulo, Brazil
dc.identifier.doi 10.1007/s00436-003-1031-2
dc.description.source Web of Science
dc.identifier.wos WOS:000220564400006



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