Phagocytosis of apoptotic cells increases the susceptibility of macrophages to infection with Coxiella burnetii phase II through down-modulation of nitric oxide production

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dc.contributor.author Zamboni, Dario Simões [UNIFESP]
dc.contributor.author Rabinovitch, Michel [UNIFESP]
dc.date.accessioned 2016-01-24T12:37:04Z
dc.date.available 2016-01-24T12:37:04Z
dc.date.issued 2004-04-01
dc.identifier http://dx.doi.org/10.1128/IAI.72.4.2075-2080.2004
dc.identifier.citation Infection and Immunity. Washington: Amer Soc Microbiology, v. 72, n. 4, p. 2075-2080, 2004.
dc.identifier.issn 0019-9567
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27684
dc.description.abstract Coxiella burnetii, the agent of Q fever in humans and coxiellosis in other mammals, is an obligate intracellular bacterium which is sheltered and multiplies within typically large phagolysosome-like replicative vacuoles (LRVs). We have previously shown that, compared with fibroblasts, mouse resident peritoneal macrophages control the development of LRVs and bacterial multiplication within these vacuoles. Earlier experiments with the nitric oxide (NO) synthase inhibitor aminoguanidine (AG) revealed that the control is exerted by NO induced by the bacteria. We report here that phagocytosis of apoptotic-like, but not of aldehyde-killed, lymphocytes by the macrophages reduced the production of NO induced by the bacteria and increased the development of LRVs and, therefore, the total bacterial load in the cultures. Experiments with macrophages from mice deficient for inducible NO synthase (iNOS(-)/(-)) confirmed the involvement of NO in the control of infection, since neither apoptotic lymphocytes nor AG affected the development of LRVs in these phagocytes. Since macrophages are important for the clearance of apoptotic bodies and C burnetii is able to induce apoptosis in human monocytes, the phenomenon shown here may be biologically relevant to the development of Q fever and coxiellosis. en
dc.format.extent 2075-2080
dc.language.iso eng
dc.publisher Amer Soc Microbiology
dc.relation.ispartof Infection and Immunity
dc.rights Acesso aberto
dc.title Phagocytosis of apoptotic cells increases the susceptibility of macrophages to infection with Coxiella burnetii phase II through down-modulation of nitric oxide production en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.identifier.file WOS000220481600027.pdf
dc.identifier.doi 10.1128/IAI.72.4.2075-2080.2004
dc.description.source Web of Science
dc.identifier.wos WOS:000220481600027



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