High expression of myeloid-related proteins 8 and 14 characterizes an inflammatorily active but ineffective response of macrophages during leprosy

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dc.contributor.author Sunderkotter, C. H.
dc.contributor.author Tomimori-Yamashita, J.
dc.contributor.author Nix, V
dc.contributor.author Maeda, S. M.
dc.contributor.author Sindrilaru, A.
dc.contributor.author Mariano, Mario [UNIFESP]
dc.contributor.author Sorg, C.
dc.contributor.author Roth, J.
dc.date.accessioned 2016-01-24T12:37:04Z
dc.date.available 2016-01-24T12:37:04Z
dc.date.issued 2004-04-01
dc.identifier http://dx.doi.org/10.1111/j.0019-2805.2004.01836.x
dc.identifier.citation Immunology. Oxford: Blackwell Publishing Ltd, v. 111, n. 4, p. 472-480, 2004.
dc.identifier.issn 0019-2805
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27682
dc.description.abstract Macrophages are decisive cells for the course of leprosy as they phagocytose Mycobacterium leprae and have the potential to influence the specific immune response. Expression and release of the myeloid-related protein (MRP) 8 and MRP14 (S100A8 and S100A9) characterize a proinflammatory subtype of macrophage that is prominent in, for example, murine infection with lack of a T helper 1 cell response and in certain highly active chronic inflammations of mice and humans. We investigated cutaneous biopsies of the different forms of leprosy (41 untreated patients) including leprosy reaction type 1 (reversal reaction) and type 2 (erythema nodosum leprosum) (n = 18) for expression of MRP8 and MRP14 by subtypes of macrophages. Concomitantly we determined serum levels of MRP8 and MRP14 by sandwich enzyme-linked immunosorbent assay. Expression of MRP8 and MRP14 by CD68-positive macrophages was low in tuberculoid leprosy and rose significantly in borderline tuberculoid leprosy and especially in multibacillary forms, there being expressed by mycobacteria-loaded foam cells. A significant rise of MRP8 and MRP14 expression also occurred in lepra reactions compared to the corresponding non-reactional forms. in type 2 reactions this additional increase was associated with a sigificant elevation of serum levels. in type 1 it was associated with expression of MRP8 and MRP14 by epitheloid and giant cells, which so far were considered not to express both proteins. in conclusion, we present evidence that the two prominent proteins MRP8 and MRP14 can be re-expressed in vivo by tissue macrophages in chronic infection, that their increased expression is characteristic for a macrophage subtype associated with high inflammatory but low antimycobacterial activity in the absence of a T helper 1 response, and that their significant rise in serum during erythema nodosum leprosum bears diagnostic and pathophysiological relevance. en
dc.format.extent 472-480
dc.language.iso eng
dc.publisher Blackwell Publishing Ltd
dc.relation.ispartof Immunology
dc.rights Acesso aberto
dc.title High expression of myeloid-related proteins 8 and 14 characterizes an inflammatorily active but ineffective response of macrophages during leprosy en
dc.type Artigo
dc.contributor.institution Univ Ulm
dc.contributor.institution Univ Munster
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Ulm, Dept Dermatol, D-89081 Ulm, Germany
dc.description.affiliation Univ Munster, Inst Expt Dermatol, D-4400 Munster, Germany
dc.description.affiliation Univ Munster, Dept Dermatol, D-4400 Munster, Germany
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Dermatol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Dermatol, São Paulo, Brazil
dc.identifier.doi 10.1111/j.0019-2805.2004.01836.x
dc.description.source Web of Science
dc.identifier.wos WOS:000220547100016



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