The Th1/Th2 immune-type response of the recurrent aphthous ulceration analyzed by cDNA microarray
Borra, R. C.
Andrade, P. M.
Smirnova, A. S.
Is part ofJournal of Oral Pathology & Medicine
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BACKGROUND: the reduced ability to activate oral tolerance plays a role in the pathogenesis of some gastrointestinal inflammatory diseases. This activation may reflect a preferential reduction of a T-helper (Th)2- or Th3-type response. in recurrent aphthous ulceration (RAU), genetic and environmental factors may contribute to low tolerance, permitting a cytotoxic reaction against the oral epithelium. the cytokine profile has not permitted the definition of RAU as resulting from enhanced Th1 or Th2 responses. A cDNA microarray study would allow the identification of differentially expressed genes and provide a basis for classification of the immune response.METHODS: the cDNA from 29 samples of aphthae and from 11 samples of normal mucosa from aphthae-free volunteers were hybridized on microarray membranes with 1176 genes.RESULTS: Forty-one differentially expressed genes were identified, and a higher expression level of the Th1 gene cluster in RAU was found.CONCLUSIONS: Microarrays permitted us definition of the gene expression profile of the lesion and identify an increased Th1 activity in RAU lesions.
CitationJournal of Oral Pathology & Medicine. Copenhagen: Blackwell Munksgaard, v. 33, n. 3, p. 140-146, 2004.
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