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dc.contributor.authorSilveira, TGV
dc.contributor.authorTakahashi, H. K.
dc.contributor.authorStraus, A. H.
dc.identifier.citationParasitology. New York: Cambridge Univ Press, v. 127, p. 449-456, 2003.
dc.description.abstractThe immunolocalization of Leishmania (Viannia) braziliensis stage-specific antigens recognized by mAbs was analysed by transmission electron microscopy. the antigen recognized by mAb SST-2 was present at the surface of promastigotes, including the flagellum and flagellar pocket. the reactivity of SST-2 with isolates of different serodemes showed a pronounced microheterogencity in terms of the number of reactive bands within the low molecular weight range from 24 to 33 kDa. the 180 kDa glycoprotein recognized by mAb SST-3 was present only in the flagellar membrane. SST-3 also recognized multiple discrete bands from 160 to 200 kDa, as observed in several serodemes. in contrast, mAb SST-4, which recognizes a 98 kDa antigen, showed weak labelling on the promastigote surface by transmission electron microscopy and indirect immunofluorescence. Based on Western blotting, indirect immunofluorescence, and solid-phase radioimmunoassay, the antigens recognized by mAbs SST-2, SST-3 and SST-4 were present in all L. (V.) braziliensis analysed, from 7 different serodemes.en
dc.publisherCambridge Univ Press
dc.rightsAcesso restrito
dc.subjectLeishmania (Viannia) braziliensisen
dc.subjectmonoclonal antibodyen
dc.subjectspecific antigensen
dc.titleImmunolocalization of Leishmania (Viannia) braziliensis membrane antigens recognized by mAbs SST-2, SST-3, and SST-4en
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, SP, Brazil
dc.description.sourceWeb of Science

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