Are there any structural alterations in a neovascularized jejunal segment by omentoenteropexy? A histologic and immunohistochemistry study

Abstract

Background/Purpose: Although the bowel neovascularization by omentoenteropexy seems to be a good alternative to bowel lengthening, there were no rigorous analyses about its histologic structure and intrinsic innervation. the aim of this study was a histologic and immunohistochemical analysis of a neovascularized jejune segment (NJS) by omentoenteropexy.Methods: An NJS was done by omentoenteropexy in 13 Wistar rats. Seven weeks afterward, the mesentery of the NJS was ligated and divided. the NJS was separated from the normal jejunum by proximal and distal complete sections. End-to-end anatomosis was done between NJS and normal jejunum to restore bowel continuity. Twenty-six rats were sham operated. At 10 weeks, specimens were taken, and H&E, S-100 protein, and neuron-specific enolase staining were done. the muscle layer thickness, mucosae layer, and myenteric plexus were analyzed. the statistical tests were the Mann-Whitney test, Friedman's test, and Spearman's correlation.Results: There was a significant increase in NJS diameter. the muscle layers and mucosa were greater in the study group. the average number of neurons per intestinal ganglion was significantly fewer in the NJS.Conclusions: Omentoenteropexy provoked an increase in the diameter of NJS and contributed toward a reduction of the number of neurons in the myenteric plexus of NJS.