HSP70 induced by hyperosmotic stress partially protects LLC-PK1 cells against nephrotoxic drugs
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2003-07-01
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Leitao, A. A.
Leite, C. A.
Santos, B. C.
Boim, M. A.
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Accumulation of HSP70 is related to the cytoprotection. It was evaluated whether hyperosmotic stress induces HSP70 accumulation in LLC-PK1 cells, and protects cells against toxicity provoked by cisplatin (Cis) and cyclosporine A (CyA). Cells were maintained in isosmotic (Iso) or hyperosmotic (H) culture medium for 24 h and then exposed to Cis or CyA for an additional period of 12 or 24 h (groups H+Cis and H+CyA). the H medium did not induce cell death and increased both HSP70 mRNA and protein levels, suggesting a role in cell adaptation to H condition. H medium produced partial cytoprotection against Cis and CyA compared with control cells. Despite the cytoprotection, there was a reduction in HSP70 mRNA and protein levels in H+Cis group. in contrast, the H+CyA group presented high levels of HSP70 mRNA and protein. the induction of HSP70 by H medium was associated with tolerance of LLC-PK1 cells against Cis and CyA cytotoxicity but this protection was induced by different mechanisms and depended on the characteristics of the drug used. Copyright (C) 2003 S. Karger AG, Basel.
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Nephron Experimental Nephrology. Basel: Karger, v. 94, n. 3, p. E94-E102, 2003.