Pravastatin protection from cold stress in myocardium of rats

Pravastatin protection from cold stress in myocardium of rats

Author Bombig, Maria Teresa Nogueira Autor UNIFESP Google Scholar
Ferreira, Celso Autor UNIFESP Google Scholar
Mora, Osvaldo Autor UNIFESP Google Scholar
Soares, Joaquim Domingos Autor UNIFESP Google Scholar
Povoa, Rui Autor UNIFESP Google Scholar
Luna, Braulio Autor UNIFESP Google Scholar
Simoes, Manoel de Jesus Autor UNIFESP Google Scholar
Murad, Neif Autor UNIFESP Google Scholar
Costa, Andrea Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Alfenas
Abstract The aim of this research was to evaluate the possible protective effect of pravastatin on ultrastructural alterations induced by cold stress in the myocardium of rats.Sixteen EPM-Wistar rats (Rattus norvegicus albinus) were used and distributed into four groups: 1) control; 2) pravastatin; 3) cold stress, and 4) pravastatin + cold stress. A daily oral dose of 10 mg/kg of weight of pravastatin was administered to each rat in groups 2 and 4 for 15 days. the stress induced by cold was obtained by keeping the group 3 and 4 rats in a freezer at -8degreesC for 4 hours. the animals were killed and the heart and fragments of the left ventricles (LV) were removed and processed prior to conducting electron microscopic analysis.The ultrastructural alterations in cardiomyocytes were quantified through the number of mitochondrial cristae pattern (cristalysis). the group subjected only to cold stress showed a significant increase in cristalysis (391.9) when compared with control group (42.0). in the cold stress and pravastatin pretreatment group, a statistically significant (96.9)*, P<0.05 cristalysis reduction was observed when compared with cold stress group. the mitochondrial cristalysis profiles of the control and pravastatin groups were 42.0 and 65.7, respectively.Cold stress induced a significant increase in the rat e of mitochondrial cristalysis. in the group that received pravastatin and was exposed to cold stress, the drug protected the LV cardiomyocytes. This fact was confirmed by a reduction mitoctiondrial cristalysis pattern. (Jpn Heart J 2003; 44: 243-255).
Keywords cold stress
pravastatin
cardiac mitochondria
Language English
Date 2003-03-01
Published in Japanese Heart Journal. Tokyo: Japan Heart Journal, Second Dept of Internal Med, v. 44, n. 2, p. 243-255, 2003.
ISSN 0021-4868 (Sherpa/Romeo, impact factor)
Publisher Japan Heart Journal, Second Dept of Internal Med
Extent 243-255
Origin http://dx.doi.org/10.1536/jhj.44.243
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000182627300010
URI http://repositorio.unifesp.br/handle/11600/27160

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