A specific subtype C of human immunodeficiency virus type 1 circulates in Brazil

Date
2003-01-03Author
Soares, M. A.
Oliveira, T. de
Brindeiro, R. M.
Diaz, Ricardo Sobhie [UNIFESP]
Sabino, E. C.
Brigido, L.
Pires, I. L.
Morgado, M. G.
Dantas, M. C.
Barreira, D.
Teixeira, P. R.
Cassol, S.
Tanuri, A.
Brazilian Network Drug Reistance S
Type
ArtigoISSN
0269-9370Is part of
AidsDOI
10.1097/01.aids/0000042595.39174.a4Metadata
Show full item recordAbstract
Objective: To characterize the subtype C strains of HIV type I that circulate in Brazil, especially those originated from the southern part of the country.Design and methods: One hundred and twelve HIV-1-positive subjects had their plasma viral RNA extracted. Protease (PR) and reverse transcriptase (RT) genomic regions were polymerase chain reaction-amplified and sequenced for subtype determination. Subtype C strains were selected and compared to other strains of this subtype from the database, and specific amino acid signature patterns were searched.Results: Brazilian subtype C viruses form a very strong monophyletic group when compared to subtype C viruses from other countries and presented specific signature amino acids. Recombinants between subtype C and B viruses have been documented in areas of co-circulation. the incidence of primary PR and RT inhibitor resistance mutations in drug-naive subjects was observed. An increasing number of secondary resistance mutations was also seen, some of which are characteristic of subtype C-related sequences.Conclusions: Introduction of subtype C of HIV-1 in Brazil was likely a single event of one or a mixture of similarly related strains. Recombination between subtype C and B viruses is an ongoing process in the country. Primary and secondary drug resistance mutations were observed, although some of the secondary mutations could be associated with subtype C molecular signatures. Subtype-specific polymorphisms of PR and RT sequences found in this subtype C Brazilian variant might influence this emergence and have an impact on HIV treatment and on vaccine development in the country. (C) 2003 Lippincott Williams Wilkins.
Citation
Aids. Philadelphia: Lippincott Williams & Wilkins, v. 17, n. 1, p. 11-21, 2003.Keywords
subtype Cdrug resistance mutations
subtype polymorphism
HIV in primary infection
signature sequence
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