Mast cells are present in epithelial layers of different tissues of the mollusc Anomalocardia brasiliana. in situ characterization of heparin and a correlation of heparin and histamine concentration

Mast cells are present in epithelial layers of different tissues of the mollusc Anomalocardia brasiliana. in situ characterization of heparin and a correlation of heparin and histamine concentration

Author Santos, E. A. Google Scholar
Rocha, LRM Google Scholar
Pereira, NML Google Scholar
Andrade, GPV Google Scholar
Nader, H. B. Google Scholar
Dietrich, C. P. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande Norte
Abstract Heparin, other sulphated glycosaminoglycans and histamine were extracted from various dissected organs of Anomalocardia brasiliana, a mollusc from the South Atlantic, and quantified. A good correlation between heparin and histamine content was found in the labial palp, intestine, ctenidium, mantle and foot tissues. the tissue location of metachromatic cells, putatively containing heparin, was identified histologically with Alcian Blue, Toluidine Blue, Masson trichrome, Haematoxylin - Eosin and PAS. Except for the foot, cells containing metachromatic granules were found in the epithelium surfaces of all the organs analysed. An in situ identification of heparin using nitrous acid and heparinase degradation has established unequivocally the presence of this compound in the metachromatic cells. the location of 'mast-like' cells at the epithelium surface of mollusc tissues exposed to the environment are very similar to the distribution of mammalian and other vertebrate mast cells and gives support to the suggestion for a role of mast cells in defense mechanisms.
Language English
Date 2002-11-01
Published in Histochemical Journal. Dordrecht: Kluwer Academic Publ, v. 34, n. 11-12, p. 553-558, 2002.
ISSN 0018-2214 (Sherpa/Romeo, impact factor)
Publisher Kluwer Academic Publ
Extent 553-558
Origin http://dx.doi.org/10.1023/A:1026020930902
Access rights Closed access
Type Article
Web of Science ID WOS:000185680200005
URI http://repositorio.unifesp.br/handle/11600/27031

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