Characterization of four substrates emphasizes kinetic similarity between insect and human C-domain angiotensin-converting enzyme

Characterization of four substrates emphasizes kinetic similarity between insect and human C-domain angiotensin-converting enzyme

Author Hens, K. Google Scholar
Vandingenen, A. Google Scholar
Macours, N. Google Scholar
Baggerman, G. Google Scholar
Carmona, Adriana Karaoglanovic Autor UNIFESP Google Scholar
Schoofs, L. Google Scholar
De Loof, A. Google Scholar
Huybrechts, R. Google Scholar
Institution Catholic Univ Louvain
Universidade Federal de São Paulo (UNIFESP)
Abstract Angiotensin converting enzyme (ACE) was already discovered in insects in 1994, but its physiological role is still enigmatic. We have addressed this problem by purifying four new ACE substrates from the ovaries of the grey fleshfly, Neobellieria bullata . Their primary structures were identified as NKLKPSQWISLSD (Neb -ODAIF- 1(1-13) ), NKLKPSQWI (Neb -ODAIF- 1(1-9) ), SLKPSNWLTPSE (Neb -ODAIF- 2) and LEQIYHL. Database analysis showed significant homology with amino acid sequence stretches as present in the N-terminal part of several fly yolk proteins. An antiserum raised against Neb -ODAIF-1(1-9) immunostained one out of three yolk protein bands of SDS/PAGE-separated fly haemolymph and egg homogenate, thus confirming that these peptides originate from a yolk protein gene product. Kinetic analysis of these peptides and of the peptides Neb -ODAIF and Neb -ODAIF- 1(1-7) with insect ACE and human ACE show both similar and unique properties for insect ACE as compared with human C-domain ACE.
Keywords ACE kinetics
domain specific substrates
insect physiology
reproduction
Language English
Date 2002-07-01
Published in European Journal of Biochemistry. Oxford: Blackwell Publishing Ltd, v. 269, n. 14, p. 3522-3530, 2002.
ISSN 0014-2956 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing Ltd
Extent 3522-3530
Origin http://dx.doi.org/10.1046/j.1432-1033.2002.03043.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000176920600021
URI http://repositorio.unifesp.br/handle/11600/26901

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