BmTI antigens induce a bovine protective immune response against Boophilus microplus tick

Show simple item record Andreotti, R. Gomes, A. Malavazi-Piza, K. C. Sasaki, S. D. Sampaio, CAM Tanaka, A. S. 2016-01-24T12:33:18Z 2016-01-24T12:33:18Z 2002-03-01
dc.identifier.citation International Immunopharmacology. Amsterdam: Elsevier B.V., v. 2, n. 4, p. 557-563, 2002.
dc.identifier.issn 1567-5769
dc.description.abstract Boophilus microplus trypsin inhibitors (BmTIs) present in larvae were preliminarily characterized as active proteins, approximately 10-18 kDa, by SDS-PAGE. BmTIs showed trypsin inhibitory activity on reverse zymography containing gelatin (0.03%) and also inhibited others serine proteinases (human neutrophil elastase and human plasma kallikrein). Bos indicus, Nelore breed calves, previously sensitized with BmTIs and challenged with tick larvae (20,000 larvae/animal), showed 72.8% efficacy to interfere in tick development with 69.7% and 71.3% reduction of both tick number and egg weight, respectively. Cattle BmTIs antiserum titer was approximately 1:8000. the maximum level of BmTIs antibody production was detected 40 days after the first immunization by ELISA. Our preliminary results suggest that B. microplus serine proteinase inhibitors may play a role in the tick larvae fixation and feeding processes. Therefore, the development of antibodies against BmTIs might impair the normal parasitism. (C) 2002 Elsevier Science B.V. All rights reserved. en
dc.format.extent 557-563
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof International Immunopharmacology
dc.rights Acesso restrito
dc.subject serine proteinase inhibitor en
dc.subject Boophilus microplus en
dc.subject tick en
dc.subject bovine en
dc.subject vaccine en
dc.title BmTI antigens induce a bovine protective immune response against Boophilus microplus tick en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.description.affiliation UNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliation Embrapa Gado Corte, BR-79002970 Campo Grande, MS, Brazil
dc.description.affiliationUnifesp UNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/S1567-5769(01)00203-X
dc.description.source Web of Science
dc.identifier.wos WOS:000174814500015


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