BmTI antigens induce a bovine protective immune response against Boophilus microplus tick

BmTI antigens induce a bovine protective immune response against Boophilus microplus tick

Author Andreotti, R. Google Scholar
Gomes, A. Google Scholar
Malavazi-Piza, K. C. Google Scholar
Sasaki, S. D. Google Scholar
Sampaio, CAM Google Scholar
Tanaka, A. S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Abstract Boophilus microplus trypsin inhibitors (BmTIs) present in larvae were preliminarily characterized as active proteins, approximately 10-18 kDa, by SDS-PAGE. BmTIs showed trypsin inhibitory activity on reverse zymography containing gelatin (0.03%) and also inhibited others serine proteinases (human neutrophil elastase and human plasma kallikrein). Bos indicus, Nelore breed calves, previously sensitized with BmTIs and challenged with tick larvae (20,000 larvae/animal), showed 72.8% efficacy to interfere in tick development with 69.7% and 71.3% reduction of both tick number and egg weight, respectively. Cattle BmTIs antiserum titer was approximately 1:8000. the maximum level of BmTIs antibody production was detected 40 days after the first immunization by ELISA. Our preliminary results suggest that B. microplus serine proteinase inhibitors may play a role in the tick larvae fixation and feeding processes. Therefore, the development of antibodies against BmTIs might impair the normal parasitism. (C) 2002 Elsevier Science B.V. All rights reserved.
Keywords serine proteinase inhibitor
Boophilus microplus
tick
bovine
vaccine
Language English
Date 2002-03-01
Published in International Immunopharmacology. Amsterdam: Elsevier B.V., v. 2, n. 4, p. 557-563, 2002.
ISSN 1567-5769 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 557-563
Origin http://dx.doi.org/10.1016/S1567-5769(01)00203-X
Access rights Closed access
Type Article
Web of Science ID WOS:000174814500015
URI http://repositorio.unifesp.br/handle/11600/26790

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