Reactivity of MEST-1 (antigalactofuranose) with Trypanosoma cruzi, glycosylinositol phosphorylceramides (GIPCs): Immunolocalization of GIPCs in acidic vesicles of epimastigotes
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2001-09-01
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Suzuki, Erika [UNIFESP]
Mortara, Renato A. [UNIFESP]
Takahashi, Helio K. [UNIFESP]
Straus, Anita H. [UNIFESP]
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Using confocal microscopy, MEST-1-positive immunofluorescence was observed within various Trypanosoma cruzi forms, except in cell-derived trypomastigotes. Glycosylinositol phosphorylceramides were identified by thin-layer chromatography immunostaining as the antigens recognized by MEST-1 in these parasites. in epimastigotes, labeling of MEST-1 coincided with acidic vesicles, indicating an internal localization of these glycoconjugates.
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Clinical and Diagnostic Laboratory Immunology. Washington: Amer Soc Microbiology, v. 8, n. 5, p. 1031-1035, 2001.