Intrauterine food restriction as a determinant of nephrosclerosis

Date
2001-03-01Author
Lucas, SRR
Miraglia, S. M.
Gil, F. Z.
Coimbra, T. M.
Type
ArtigoISSN
0272-6386Is part of
American Journal of Kidney DiseasesDOI
10.1053/ajkd.2001.22088Metadata
Show full item recordAbstract
We previously showed that 8-month-old rats subjected to a 50% intrauterine food restriction had a decreased number of nephrons with increased glomerular diameter, which suggests compensatory hypertrophy, Hypertrophy could be the early event of glomerular damage. in this study, we extended our investigation and performed functional, morphological, and immunohistochemical evaluations in 3- and 18-month-old rats that underwent a 50% intrauterine food restriction (RT3 and RT18, respectively) and age-matched control rats (C3 and C18, respectively). Our findings showed that glomerular filtration rate was significant decreased in RT18 rats (2.42 +/- 0.15 mL/min/kg; n = 28; P < 0.05) compared with C18 control rats (4.19 +/- 0.10 ml/min/kg; P < 0.05) and the percentage of glomeruli with sclerosis was greater in RT18 rats (13.01% +/- 2.95%; n = 9; P < 0.01) than in C18 rats (2.71% +/- 0.35%; n = 6), RT18 rats also showed more intense tubulointerstitial lesions and immunohistochemical alterations in the renal cortex. Immunohistochemical studies showed increased fibronectin and desmin expression in glomeruli and tubulointerstitium and increased vimentin and Lu-smooth muscle actin in the tubulointerstitial area from the renal cortex of RT18 rats (P < 0.05), Desmin was also increased at the edge of glomeruli from RT18 rats, suggesting podocyte injury. Our data show that when food restriction is imposed during pregnancy, permanent damage occurs in the kidney of the offspring. Glomerular lesions were more severe than the tubulointerstitial damage in these animals. (C) 2001 by the National Kidney Foundation, Inc.
Citation
American Journal of Kidney Diseases. Philadelphia: W B Saunders Co, v. 37, n. 3, p. 467-476, 2001.Keywords
food restrictionglomerular hypertrophy
glomerulosclerosis
tubulointerstitial lesions
nephrosclerosis
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