Genetic characterization of adenovirus strains isolated from patients with acute conjunctivitis in the city of São Paulo, Brazil

Date
2000-05-01Author
Tanaka, K.
Itoh, N.
Saitoh-Inagawa, W.
Uchio, E.
Takeuchi, S.
Aoki, K.
Soriano, E.
Nishi, M.
Junior, R. B.
Harsi, C. M.
Tsuzuki-Wang, L.
Durigon, Edison Luiz
Stewien, Klaus Eberhard
Ohno, S.
Type
ArtigoISSN
0146-6615Is part of
Journal of Medical VirologyDOI
10.1002/(SICI)1096-9071(200005)61:1<143Metadata
Show full item recordAbstract
Genome analysis was carried out on adenovirus strains isolated from patients with acute follicular conjunctivitis in the city of São Paulo, Brazil. Eighteen conjunctival scrapings, collected between December 1993 and March 1994, were analyzed by two methods: a combination of polymerase chain reaction with restriction fragment length polymorphism and viral DNA restriction analysis, carried out using 10 restriction endonucleases: BamHI, BglI, BglII, HindIII, KpnI, SacI, SalI, SmaI, XbaI, and XhoI. Among 11 adenovirus detected by cell culture isolation, nine were Ad8, and two were Ad7. By restriction analysis the Ad8 isolates were typed as two new variants-Ad8/D11 (seven of nine samples) and Ad8/D12 (two of nine samples). Ad7 isolates were identified as a subtype of the widespread genome type Ad7b and the virulent type Ad7h, a predominant genome type circulating in Argentina, Chile, and Uruguay but absent in Brazil until 1991. J. Med. Virol 61:143-149, 2000.
Citation
Journal of Medical Virology. New York: Wiley-liss, v. 61, n. 1, p. 143-149, 2000.Keywords
Brazilacute conjunctivitis
adenovirus
new genome types
polymerase chain reaction with restriction fragment length polymorphism
genome typing
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