A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana
Chavante, S. F.
Santos, E. A.
Oliveira, F. W.
Dietrich, C. P.
Nader, H. B.
Is part ofInternational Journal of Biological Macromolecules
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With the aid of heparinase and heparitinases from Flavobacterium heparinum and C-13 and H-I NMR spectroscopy it was shown that the heparan sulphate isolated from the brine shrimp Artemia franciscana exhibits structural features intermediate between those of mammalian heparins and heparan sulphates. These include an unusually high degree of N-sulphation with corresponding very low degree of N-acetylation), a relatively high content of iduronic acid residues (both unsulphated and 2-O-sulphated) and a relatively low degree of 6-O-sulphation of the glucosamine residues. the major sequences (glucuronic acid-->N-sulphated glucosamine and glucuronic acid--> N,6-disulphated glucosamine) are most probably arranged in blocks. Although exhibiting negligible anticlotting activity in the APTT and anti-factor Xa assays the A. franciscana heparan sulphate has a high heparin cofactor-II activity (about 1/3 that of heparin). (C) 2000 Elsevier Science B.V. All rights reserved.
CitationInternational Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 27, n. 1, p. 49-57, 2000.
Keywordsbrine shrimp heparan sulphate
crustacean heparan sulphate
heparin cofactor-II activity
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