A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana

Date
2000-03-16Author
Chavante, S. F.
Santos, E. A.
Oliveira, F. W.
Guerrini, M.
Torri, G.
Casu, B.
Dietrich, C. P.
Nader, H. B.
Type
ArtigoISSN
0141-8130Is part of
International Journal of Biological MacromoleculesDOI
10.1016/S0141-8130(99)00114-2Metadata
Show full item recordAbstract
With the aid of heparinase and heparitinases from Flavobacterium heparinum and C-13 and H-I NMR spectroscopy it was shown that the heparan sulphate isolated from the brine shrimp Artemia franciscana exhibits structural features intermediate between those of mammalian heparins and heparan sulphates. These include an unusually high degree of N-sulphation with corresponding very low degree of N-acetylation), a relatively high content of iduronic acid residues (both unsulphated and 2-O-sulphated) and a relatively low degree of 6-O-sulphation of the glucosamine residues. the major sequences (glucuronic acid-->N-sulphated glucosamine and glucuronic acid--> N,6-disulphated glucosamine) are most probably arranged in blocks. Although exhibiting negligible anticlotting activity in the APTT and anti-factor Xa assays the A. franciscana heparan sulphate has a high heparin cofactor-II activity (about 1/3 that of heparin). (C) 2000 Elsevier Science B.V. All rights reserved.
Citation
International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 27, n. 1, p. 49-57, 2000.Keywords
brine shrimp heparan sulphatestructure
heparan sulphate
Artemia franciscana
crustacean heparan sulphate
anticlotting activities
heparin cofactor-II activity
Collections
- EPM - Artigos [17701]
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