Characterization of a tissue kallikrein inhibitor isolated from Bauhinia bauhinioides seeds: inhibition of the hydrolysis of kininogen related substrates

Characterization of a tissue kallikrein inhibitor isolated from Bauhinia bauhinioides seeds: inhibition of the hydrolysis of kininogen related substrates

Author Oliva, MLV Google Scholar
Mendes, C. R. Google Scholar
Juliano, M. A. Google Scholar
Chagas, JR Google Scholar
Rosa, J. C. Google Scholar
Greene, L. J. Google Scholar
Sampaio, M. U. Google Scholar
Sampaio, CAM Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Trypsin inhibitors were purified from a saline extract of Bauhinia bauhinioides seeds by ion-exchange column chromatography on DEAE-Sephadex, gel filtration on Superose 12 column, Mono Q ion-exchange chromatography or, alternatively, by affinity chromatography on trypsin-Sepharose. Both B. bauhinioides isolated inhibitors, BbTI-I and BbTI-II, inhibit trypsin being the dissociation constant 0.6 and 0.36 nM, respectively. BbTI-II only inhibits porcine pancreatic kallikrein hydrolysis of H-Pro-Phe-Arg-AMC (K-i 2.0 nM); the bradykinin-containing sequence LGMISLMKRPPGFSPFRSSRI-NH2 and the two kininogen related flanking quenched substrates Abz-MISLMKRP-EDDnp (K-i 2.0 nM) and Abz-FRSSRQ-EDDnp (K-i 2.5 nM). (C) 1999 Published by Elsevier Science B.V. All rights reserved.
Keywords kallikrein
serine proteinase inhibitor
blood clotting enzymes
tissue kallikrein inhibitor
Language English
Date 1999-12-01
Published in Immunopharmacology. Amsterdam: Elsevier B.V., v. 45, n. 1-3, p. 163-169, 1999.
ISSN 0162-3109 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 163-169
Origin http://dx.doi.org/10.1016/S0162-3109(99)00075-2
Access rights Closed access
Type Article
Web of Science ID WOS:000084080100027
URI http://repositorio.unifesp.br/handle/11600/26187

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