Glycosaminoglycans in components of the rabbit eye: synthesis and characterization

Glycosaminoglycans in components of the rabbit eye: synthesis and characterization

Author Goes, R. M. Google Scholar
Laicine, E. M. Google Scholar
Porcionatto, M. A. Google Scholar
Nader, H. B. Google Scholar
Haddad, A. Google Scholar
Institution Universidade de São Paulo (USP)
UNESP
Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose. To trace the eye components involved in proteoglycan synthesis and to characterize the sulfated glycosaminoglycans which are associated to these macromolecules.Methods. Sodium [S-35]-sulfate was injected intravitreally and the rabbits were killed at different time intervals after the injection. the glycosaminoglycans of choroid, ciliary body, cornea, iris, lens capsule, retina and sclera were extracted and processed for estimations of their specific activities, and for electrophoresis plus autoradiography with or without previous treatment with specific enzymes. in addition, methacrylate sections of the eyes were analysed by autoradiography.Results. the peak of specific activities of the glycosaminoglycans of all eye components occurred at 2 days after the intravitreal injection of [S-35]-sulfate. the autoradiography of the agarose gels revealed three types of glycosaminoglycans, namely, heparan-, chondroitin- and dermatan sulfate, only in the retina. the other eye components contained heparan sulfate and either chondroitin or dermatan sulfate. Tissue autoradiography together with the biochemical techniques contributed to unravel the origin of the glycosaminoglycans in the eye components.Conclusions. the results of the present investigation have shown that heparan sulfate, contrasting to chondroitin sulfate and dermatan sulfate, is synthesized in all eye components studied and that the glycosaminoglycan composition differs according to the tissue of origin.
Keywords glycosaminoglycans
eye
heparan sulfate
condroitin sulfate
dermatan sulfate
rabbit
rate of synthesis
Language English
Date 1999-08-01
Published in Current Eye Research. Lisse: Swets Zeitlinger Publishers, v. 19, n. 2, p. 146-153, 1999.
ISSN 0271-3683 (Sherpa/Romeo, impact factor)
Publisher Swets Zeitlinger Publishers
Extent 146-153
Origin http://dx.doi.org/10.1076/ceyr.19.2.146.5324
Access rights Closed access
Type Article
Web of Science ID WOS:000082004600008
URI http://repositorio.unifesp.br/handle/11600/26127

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