Identification and characterization of a sialidase released by the salivary gland of the hematophagous insect Triatoma infestans

Date
1998-09-18Author
Amino, Rogerio [UNIFESP]
Marques-Porto, Rafael [UNIFESP]
Chammas, Roger [UNIFESP]
Egami, Mizue Imoto [UNIFESP]
Schenkman, Sergio [UNIFESP]
Type
ArtigoISSN
0021-9258Is part of
Journal of Biological ChemistryDOI
10.1074/jbc.273.38.24575Metadata
Show full item recordAbstract
Sialidases (EC 3.2.1.18) are commonly found in viruses, bacteria, fungi, protozoa, and vertebrates, but not in invertebrates. We have previously reported the presence of a new siaIidase activity in the gut of exclusively hematophagous insects of the Triatoma genus, which transmit Chagas' disease (Amino, R., Acosta, A, Morita, O. M., Chioccola, V. L. P., and Schenkman, S. (1995) Glycobiology 5, 625-631). Here we show that this sialidase is present in the salivary gland of Triatoma infestans, and it is released with the saliva during the insect bite. the sialidase was purified to homogeneity (>5000 times) to a specific activity of more than 20 units/mg. It elutes from a gel filtration column with a volume corresponding to the size of 33 kDa, and it migrates as a single 26-kDa band in SDS-polyacrylamide gel electrophoresis, which is unusually smaller when compared with other known sialidases. T. infestans sialidase hydrolyzes preferentially alpha 2-->3-linked sialic acids at pH 4-8, with maximaI activity between pH 5.5 and 6.5, which is compatible with the optimal pH of secreted sialidases. the sialidase is competitively inhibited by 2-deoxy-2,3-dehydro-N-acetyl-neuraminic acid (K-i = 0.075 mM) and differently from many sialidases, with exception of Salmonella typhimurium sialidase, it is inhibited competitively by HEPES (K-i = 15 mM). the fact that T. infestans sialidase is released with the saliva and can hydrolyze sialyl-Lewis(x) blood groups, which are the ligands for selectins, suggests that it might have a role in the blood feeding.
Citation
Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 273, n. 38, p. 24575-24582, 1998.Collections
- EPM - Artigos [17709]
Related items
Showing items related by title, author, creator and subject.
-
Encruzilhada. Artigos dos estudantes de arte contemporânea da Unifesp (2023)
Arantes, Pedro Fiori [UNIFESP]; Pires, Catarina Silva [UNIFESP]; Souza, Larissa Silva de [UNIFESP]; Miramontes, Luana [UNIFESP]; Jean, Marci [UNIFESP]; Alcarde, Yasmin Tainá Lana de [UNIFESP]; Lima, Ingrid Dos Santos [UNIFESP]; Costa, João Henrique Lopes da [UNIFESP]; Marques, Yago Maciel [UNIFESP]; Rocha, Romario Lopes [UNIFESP]; Gomes, Gustavo Bitencourt [UNIFESP]; Dias, Camila Oliveira [UNIFESP]; Santos, Thaynná Baltazar dos[UNIFESP]; Guedes, Beatriz [UNIFESP]; Melo, Gabriella [UNIFESP]; Francesquini, Maria Sueli de Camargo [UNIFESP]; Almeida, Raphael Gomes de [UNIFESP]; Morita, Yumi [UNIFESP]; Araújo, Isadora Trindade [UNIFESP]; Santo, Micaele Silva [UNIFESP]; Rocha, Karina Braga da [UNIFESP]; Campos, Maria Eduarda [UNIFESP]; Soares, Juliane Pereira [UNIFESP]; Silva, Gabrielly A. Santiago da [UNIFESP]; Lima, Gabrielle Pereira [UNIFESP]; Marzola, Ana Paula [UNIFESP]; Mões, Gustavo [UNIFESP]; Campos, Hellen [UNIFESP]; Baba, Luccas Ryoichi Kimura [UNIFESP]; Reis, Mariana [UNIFESP]; França, Milena [UNIFESP]; Yamaguchi, Ângela Harumi [UNIFESP]; Marassi, Beatriz [UNIFESP]; Porfirio, Beatriz [UNIFESP]; Mayumi, Cristiane [UNIFESP]; Agbanusi, Gladys Uju Balbino [UNIFESP]; Calixto, Fernanda [UNIFESP]; Capelli, Thalles [UNIFESP] (Universidade Federal de São Paulo, 2023)A arte contemporânea é, acima de tudo, multidisciplinar. A experimentação e a quebra de limites são primordiais para a existência desse campo tão vibrante e diversificado que permite que os artistas se expressem de forma ...
-
I Diretriz brasileira de cardio-oncologia pediátrica da Sociedade Brasileira de Cardiologia
Seber, Adriana [UNIFESP]; Miachon, Adriana Siviero [UNIFESP]; Tanaka, Ana Cristina Sayuri; Spinola-Castro, Angela Maria [UNIFESP]; Carvalho, Antonio Carlos [UNIFESP]; Petrilli, Antonio Sergio [UNIFESP]; Macedo, Carla Renata Pacheco Donato [UNIFESP]; Nars, Clarissa Carvalho Fongaro [UNIFESP]; Terzian, Claudia Naufel [UNIFESP]; Castro Junior, Claudio Galvão de; Santos, Cleusa Cavalcanti Lapa; Guerra, Cristina Chaves dos Santos; Silva, Dafne Cardoso Bourguignon da [UNIFESP]; Bassi, Débora Ugayama; Azeka, Estela; Feitosa, Fabiana Aragão; Hamamoto, Fernando [UNIFESP]; Szarf, Gilberto [UNIFESP]; Lederman, Henrique Manoel [UNIFESP]; Rigon Junior, Humberto João; Jatene, Ieda Biscegli; Mota, Isabele Coelho Fonseca da; Perrud, Jeferson Adriano [UNIFESP]; Soares Junior, José; Gutierrez, Julián Arango; Perin, Juliana Pepe Marinho [UNIFESP]; Soares, Juliana dos Santos [UNIFESP]; Catani, Liane Hulle; Tsai, Liliana Yu [UNIFESP]; Vianna, Livia Cristina [UNIFESP]; Paiva, Marcelo Goulart; Santos, Marcelo José; Ishigai, Marcia Marcelino de Souza [UNIFESP]; Diógenes, Maria Suely Bezerra [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Piedade, Maria Tereza Castro [UNIFESP]; Parreiras, Mariana; Cypriano, Mônica [UNIFESP]; Negrini, Nilce da Silva [UNIFESP]; Campos Filho, Orlando [UNIFESP]; Figueiredo, Paula Andrade [UNIFESP]; Novaes, Paulo Eduardo [UNIFESP]; Camargo, Paulo Roberto [UNIFESP]; Maia, Priscila dos Santos [UNIFESP]; Petrilli, Renata [UNIFESP]; Arduini, Rodrigo Genaro [UNIFESP]; Gouveia, Roseane Vasconcelos [UNIFESP]; Teruya, Suzana Barbosa Miranda [UNIFESP]; Moisés, Valdir Ambrósio [UNIFESP]; Morais, Vera Lúcia Lins de (Sociedade Brasileira de Cardiologia - SBC, 2013-05-01) -
Guerras culturais em verde e amarelo
Arantes, Pedro Fiori [UNIFESP]; Okuma, André [UNIFESP]; Nascimento, Giulia dos Santos [UNIFESP]; Melo, Keyla Vasconcelos de [UNIFESP]; Lorena, Diego [UNIFESP]; Alves, Amanda [UNIFESP]; Brait, Ana Laura [UNIFESP]; Alves, Gustavo Almeida [UNIFESP]; Fernandes, Cristina Naiara [UNIFESP]; Avelino, Larissa [UNIFESP]; Paloma, Oliveira [UNIFESP]; Bertellotti, André Zaparoli [UNIFESP]; Carreño, Rebeca Nieves Inostroza [UNIFESP]; Feo, Amanda [UNIFESP]; Andrade, Bruna [UNIFESP]; Garotti, Josiane [UNIFESP]; Flauto, Larissa [UNIFESP]; Tavares, Melissa [UNIFESP]; Barbosa, Ana Beatriz Tavares [UNIFESP]; Alejarra, Melissa Maria dos Santos [UNIFESP]; Santos, Nicole Pinheiro [UNIFESP]; Dourado, Kamilla [UNIFESP]; Silva, Pamela [UNIFESP]; Goyano, Lucius [UNIFESP]; Meneses, Maria Luiza [UNIFESP]; Ferreira, Gabriela [UNIFESP]; Monteiro, Paloma [UNIFESP]; Oliveira, Marcelo Lauton de [UNIFESP]; Paula, Patrícia Pinheiro Antunes de [UNIFESP]; Santos, Isabella Mendes dos [UNIFESP]; Borges, Júlia Rodrigues [UNIFESP] (Universidade Federal de São Paulo, 2022)Queridos/as leitores/as, bem-vindos/as ao nosso campo de batalha – e de estudos. Vocês estão prestes a ler um livro perigoso. Ele fala sobre muito do que estamos vivendo no Brasil dos últimos anos, olhando esse nosso ...