Variable region structure and Staphylococcal protein A binding specificity of a mouse monoclonal IgM anti-laminin-receptor antibody
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1997-07-01
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Feijo, GCS
Sabbaga, J.
Carneiro, Celia Regina Whitaker [UNIFESP]
Brigido, M. M.
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Staphylococcal protein A is a cell wall-attached polypeptide that acts as a B-lymphocyte superantigen. This activation correlates with specific V-H gene segment usage in the B-cell receptor. B-cell receptor assembled from members of the V(H)3 family in humans, or S107 family in mice, has an intrinsic affinity for protein A. Human V(H)3-derived antibodies bind to domain D of protein A. We have characterized a mouse IgM monoclonal antibody that binds protein A. the sequencing of the variable region suggests an almost germline-encoded V-H derived from S107 family and a V kappa 8-derived V-L. the binding specificity of the monoclonal antibody was tested with various recombinant constructions derived from protein A. We show that, unlike human V(H)3-derived antibody, mouse S107-derived immunoglobulin binds to the B domain of the bacterial superantigen.
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Immunology. Oxford: Blackwell Science Ltd, v. 91, n. 3, p. 479-485, 1997.