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dc.contributor.authorPesquero, J. B.
dc.contributor.authorPesquero, J. L.
dc.contributor.authorOliveira, S. M.
dc.contributor.authorRoscher, A. A.
dc.contributor.authorMetzger, R.
dc.contributor.authorGanten, D.
dc.contributor.authorBader, M.
dc.date.accessioned2016-01-24T11:40:30Z
dc.date.available2016-01-24T11:40:30Z
dc.date.issued1996-03-07
dc.identifierhttp://dx.doi.org/10.1006/bbrc.1996.0384
dc.identifier.citationBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc Jnl-comp Subscriptions, v. 220, n. 1, p. 219-225, 1996.
dc.identifier.issn0006-291X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/25569
dc.description.abstractThe gene encoding a putative mouse bradykinin B1 receptor was cloned from a genomic library by low stringency screening. Analysis of two isolated clones revealed a region which contains an open reading frame uninterrupted by introns and encodes a 334 amino acid protein, which exhibits seven potential transmembrane domains and is 68% identical to the human and rabbit bradykinin B1 receptors, Lipopolysaccharide-treatment induces B1 receptor transcripts in the heart, liver and lung, Stable expression of the coding region in COS-7 cells resulted in high levels of binding sites for the specific B1 ligand des-Arg(10) kallidin (K-d = 1.3 nM; B-max = 51 fmol/mg protein). the rank order of affinity of the receptor for the agonists and antagonists was: des Arg(9)BKdes-Arg(9)Leu(8)BKdrs-Arg(10)kallidin much greater than Hoe-140 = bradykinin. Functional coupling of the cloned receptor was demonstrated by the dose-dependent effect of des-Arg(9)BK on the extracellular acidification rate in stably transfected COS-7 cells. This effect was not produced by bradykinin and could be blocked by the B1 antagonist des-Arg(9)Leu(8)BK. (C) 1996 Academic Press, Inc.en
dc.format.extent219-225
dc.language.isoeng
dc.publisherAcademic Press Inc Jnl-comp Subscriptions
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.rightsAcesso restrito
dc.titleMolecular cloning and functional characterization of a mouse bradykinin B1 receptor geneen
dc.typeArtigo
dc.contributor.institutionMAX DELBRUCK CTR MOLEC MED
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUNIV MUNICH
dc.contributor.institutionCELL CONTROL BIOMED LABS
dc.description.affiliationMAX DELBRUCK CTR MOLEC MED,D-13125 BERLIN,GERMANY
dc.description.affiliationESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04032062 São Paulo,BRAZIL
dc.description.affiliationUNIV FED MINAS GERAIS,ICB,DEPT PHYSIOL & BIOPHYS,BR-31270010 BELO HORIZONT,MG,BRAZIL
dc.description.affiliationUNIV MUNICH,CHILDRENS HOSP,DEPT CLIN CHEM & BIOCHEM,D-80337 MUNICH,GERMANY
dc.description.affiliationCELL CONTROL BIOMED LABS,D-82152 MARTINSRIED,GERMANY
dc.description.affiliationUnifespESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04032062 São Paulo,BRAZIL
dc.identifier.doi10.1006/bbrc.1996.0384
dc.description.sourceWeb of Science
dc.identifier.wosWOS:A1996UA75700039


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