Evaluation of recent techniques for detection of red blood cell antibodies in sera of reference samples, patients, pregnant women, and blood donors

Date
1996-01-01Author
deCastilho, L. M.
Pellegrino, J.
Bechelli, APP
LePennec, P. Y.
Mendes, N. F.
Type
ArtigoISSN
0887-8013Is part of
Journal of Clinical Laboratory AnalysisDOI
10.1002/(SICI)1098-2825(1996)10:5<250Metadata
Show full item recordAbstract
The sensitivity of the low ionic strength solution antiglobulin test (LISS-AGT), polyethylene glycol antiglobulin test (PEG-AGT), low ionic strength solution solid-phase antiglobulin test (LISS-SPAT), gel low ionic strength solution antiglobulin test (GEL-LISS), and gel papain Bst (GEL-PAP) was compared in titration studies of 460 sera containing identified IgG alloantibodies. the GEL-PAP was 100% sensitive to detect Rh antibodies, whereas the PEG-AGT was the most sensitive to detect Kell, Duffy, Kidd, Ss, and rare blood group antibodies. the better performance of PEG-AGT was especially obvious with Kell, Duffy, and Ss antibodies (S = 100%). When the sensitivity of the LISS-AGT, PEG-AGT, GEL-LISS, and GEL-PAP was evaluated in different routines, the GEL-LISS showed to be more sensitive than PEG-AGT in the detection of clinically significant antibodies. These discrepant results showed that the performance of a technique may change when it is applied as a routine. (C) 1996 Wiley-Liss, Inc.
Citation
Journal of Clinical Laboratory Analysis. New York: Wiley-liss, v. 10, n. 5, p. 250-256, 1996.Collections
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