A NEW APPROACH for the CHARACTERIZATION of POLYSACCHARIDES FROM ALGAE - PRESENCE of 4 MAIN ACIDIC POLYSACCHARIDES in 3 SPECIES of the CLASS PHAEOPHYCEA

A NEW APPROACH for the CHARACTERIZATION of POLYSACCHARIDES FROM ALGAE - PRESENCE of 4 MAIN ACIDIC POLYSACCHARIDES in 3 SPECIES of the CLASS PHAEOPHYCEA

Author Dietrich, C. P. Google Scholar
Farias, GGM Google Scholar
Deabreu, LRD Google Scholar
Leite, E. L. Google Scholar
Dasilva, L. F. Google Scholar
Nader, H. B. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNIV FED RIO GRANDE NORTE
Abstract The fractionation of acidic polysaccharides by ion exchange chromatography followed by agarose gel electrophoresis in a special buffer system made of 1,3-diaminopropane has shown that the algae of the class Phaeophycea, order Dictyotales (Dictyota mertensis and Padina gymnospora) and order Fucales (Sargassum vulgare) as well as its floaters contain 4 main acidic polysaccharides. the compounds have a distinct electrophoretic mobility and are eluted at different concentrations of NaCl from the anion exchange resin; the chemical analyses of the compounds indicate that all the 3 algae contain alginic acid, a xylofucan and 2 species of fucans with different sulfate contents. the main findings of these studies were that this class of algae contains 4 main acidic polysaccharides and that they were similar regarding the sugar composition and sulfate content when the 3 algae were compared. the findings that the acidic polysaccharides migrate as single discrete components in electrophoresis suggest that they have defined structures and as a consequence, the sugars and sulfate residues are not randomly present in the polymer chains. These observations lead also to the conclusion that the quantitative differences of the sugars present in the crude polysaccharide mixture of the 3 algae are due to the relative proportions of the 4 acidic polysaccharides.
Keywords ACIDIC POLYSACCHARIDES
ALGAE
FUCOSE
ION EXCHANGE CHROMATOGRAPHY
Language English
Date 1995-06-30
Published in Plant Science. Clare: Elsevier Sci Publ Ireland Ltd, v. 108, n. 2, p. 143-153, 1995.
ISSN 0168-9452 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 143-153
Origin http://dx.doi.org/10.1016/0168-9452(95)04142-H
Access rights Closed access
Type Article
Web of Science ID WOS:A1995RG80600004
URI http://repositorio.unifesp.br/handle/11600/25507

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account