DECREASED MUSCARINIC RECEPTOR-BINDING in RAT-BRAIN AFTER PARADOXICAL SLEEP-DEPRIVATION - AN AUTORADIOGRAPHIC STUDY
Nunes, G. P.
Nobrega, J. N.
Is part ofBrain Research
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Previous work demonstrated that paradoxical sleep deprivation (PSD) leads to a decrease in yawning behavior elicited by cholinergic agonists, suggesting that a downregulation of cholinergic muscarinic receptors may occur after PSD. More recent work using intracerebral injections of muscarinic agonists has suggested a critical role for M2 receptors in paradoxical sleep. in this study [H-3]AF-DX 384 was used to investigate the effects of PSD on M2-type cholinergic receptors throughout the brain using quantitative autoradiography. After 96 h of paradoxical sleep deprivation, [3H]AF-DX 384 binding was generally reduced throughout the brain, and significantly so in the olfactory tubercle (-20%), n. accumbens (-23%), frontal caudate-putamen (-16%), islands of Callejas (-20%), piriform cortex (-24%), lateral (-26%) and medial (-24%) septum, anteromedial (-19%), ventrolateral (-22%), and lateral geniculate (-15%) nuclei of thalamus, deep layers of the superior colliculus (-15%), entorhinal cortex (-12%) and subiculum (-23%). [H-3]AF-DX 384 binding was reduced in pontine structures, but not to a higher degree than in other brain areas. the observed downregulation of M2-type muscarinic receptors after PSD may be causally related to the previously reported decrease in cholinergically induced behaviors after PSD.
CitationBrain Research. Amsterdam: Elsevier B.V., v. 645, n. 1-2, p. 247-252, 1994.
KeywordsPARADOXICAL SLEEP DEPRIVATION
BRAIN MUSCARINIC M2 RECEPTOR
[H-3] AF-DX 384
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