CLONING and CHARACTERIZATION of A GENE for the STAGE-SPECIFIC 82-KDA SURFACE-ANTIGEN of METACYCLIC TRYPOMASTIGOTES of TRYPANOSOMA-CRUZI

CLONING and CHARACTERIZATION of A GENE for the STAGE-SPECIFIC 82-KDA SURFACE-ANTIGEN of METACYCLIC TRYPOMASTIGOTES of TRYPANOSOMA-CRUZI

Author Araya, J. E. Google Scholar
Cano, M. I. Google Scholar
Yoshida, N. Google Scholar
Dasilveira, J. F. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We have cloned and sequenced a cDNA clone coding for a metacyclic trypomastigote-specific surface glycoprotein with a molecular mass of 82 kDa (MTS-gp82). By immunoblotting and immunoprecipitation, antibodies against the recombinant protein recognized an 82-kDa protein of metacyclic trypomastigotes, without any detectable reaction towards amastigotes, epimastigotes or tissue culture-derived trypomastigotes. the insert of the MTS-gp82 cDNA done strongly hybridized with a single 2.2-kb metacyclic trypomastigote mRNA, suggesting that the steady-state levels of mRNAs for MTS-gp82 are developmentally regulated. MTS-gp82 is encoded by a multigene family whose members are distributed in several chromosomes. Sequence analysis revealed 40-56% identity at amino acid level between MTS-gp82 and members of Trypanosoma cruzi gp85/sialidase family (TSA-1, Tt34c1, SA85-1.1). MTS-gp82 showed several amino acid motifs that are characteristic of gp85/sialidase family, such as the Asp box (SxDxGxTW), the subterminal (VTVxNVFLYNR) motif and the putative GPI-anchor sequence. On the basis of its structural features, the MTS-gp82 gene could be included in the T. cruzi gp85/sialidase family, but constituting a distinct group which is preferentially expressed in metacyclic trypomastigotes.
Keywords TRYPANOSOMA CRUZI
METACYCLIC TRYPOMASTIGOTE SURFACE ANTIGEN
82-KDA GLYCOPROTEIN
GENE CLONING
MULTIGENE FAMILY
GP85/SIALIDASE SUPERFAMILY
Language English
Date 1994-05-01
Published in Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 65, n. 1, p. 161-169, 1994.
ISSN 0166-6851 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 161-169
Origin http://dx.doi.org/10.1016/0166-6851(94)90124-4
Access rights Closed access
Type Article
Web of Science ID WOS:A1994NW86200015
URI http://repositorio.unifesp.br/handle/11600/25405

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