Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

Author Silva, T.m.a. Google Scholar
Alves, L.g. Autor UNIFESP Google Scholar
Queiroz, K.c.s. de Google Scholar
Santos, M.g.l. Google Scholar
Marques, C.t. Google Scholar
Chavante, Suely Ferreira Autor UNIFESP Google Scholar
Rocha, Hugo Alexandre Oliveira Autor UNIFESP Google Scholar
Leite, E.l. Google Scholar
Institution Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Glicobiologia
Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros Naturais
Universidade Federal de São Paulo (UNIFESP)
Abstract The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-D-glucuronic acid 1-> or 4-ß-D-glucuronic acid 1 ->, substituted at C-2 with alpha-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-alpha-L-fucose 1-> units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-O-sulfation at C-3 of 4-alpha-L-fucose 1-> units is responsible for the anticoagulant activity of the polymer.
Keywords Fucan
Anticoagulant activity
Sulfated polysaccharides
Brown algae
Padina gymnospora
Language English
Date 2005-04-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 4, p. 523-533, 2005.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 523-533
Origin http://dx.doi.org/10.1590/S0100-879X2005000400005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000228962700005
SciELO ID S0100-879X2005000400005 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/2502

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