Atividade antioxidante e alterações morfológicas induzidas pelas isoflavonas da soja no útero de ratas ovariectomizadas.
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Data
2014
Autores
Teixeira, Cristiane de Paula [UNIFESP]
Orientadores
Simões, Manuel de Jesus [UNIFESP]
Tipo
Dissertação de mestrado
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A diminuicao do estrogenio na pos-menopausa leva a perda de efeitos antioxidantes, promovendo o aumento de especies reativas de oxigenio e nitrogenio, favorecendo a ocorrencia de lesoes oxidativas. Por sua vez, as isoflavonas da soja (ISO) sao conhecidas por seu alto teor em antioxidantes. Objetivo: Avaliar o balanco pro-oxidante/antioxidante e as alteracoes morfologicas no utero de ratas ovariectomizadas (OVX) sob a influencia das ISO. Metodologia: Utilizaram-se vinte ratas Wistar com 95 dias, divididas em quatro grupos: GI-Sham (fase estro);GII-OVX; GIII-OVX tratadas com extrato concentrado de ISO (150 mg/kg/dia) por gavagem; GIV-OVX tratadas por via subcutanea com 17β-estradiol (10 µg/Kg/dia). Apos trinta dias de tratamento, as ratas foram eutanasiadas, o terco distal dos cornos uterinos fixado em formol a 10% e processado para histomorfometria e imunoistoquimica para a proteina ki-67. O restante do utero foi mergulhado em nitrogenio liquido e, posteriormente, analisado para quantificacao de especies reativas de oxigenio e nitrogenio (DCF); avaliacao da capacidade antioxidante total (TAC); e deteccao dos niveis de peroxidacao dos lipideos (TBARS). Os resultados obtidos foram submetidos a analise de variancia, seguido pelo teste de Tukey-Kramer (p≤0,005). Resultados: A espessura do endometrio e miometrio, assim como a area glandular nao diferiram significantemente entre GII e GIII, porem, mostraram-se menores em relacao a GI e GIV. O grupo GIV exibiu espessura do miometrio significantemente maior que os demais grupos. A positividade dos nucleos quanto a expressao de ki-67 em ambos o epitelio glandular e o estroma endometrial foram significativamente maiores em GI e GIV comparados com GII e GIII. O tratamento com ISO revelou menores niveis de DCF e TBARS que GII, assim como TAC significantemente maior que GI e GIV. Em GIV, apesar da diminuicao significante no TBARS, nao houve diferenca significante no DCF e foi evidenciada uma diminuicao na TAC em comparacao com GII. Conclusao: As isoflavonas melhoram os niveis de antioxidantes totais e diminuem o estresse antioxidantes totais e diminuem o estresse oxidativo, sem promover efeito trofico e proliferacao celular no utero de ratas ovariectomizadas
Estrogen deficiency at menopause leads to loss of antioxidant defense, which promotes the increase of reactive oxygen/nitrogen species (ROS/RNS) and favors the oxidative stress. Meanwhile, soy isoflavones (ISO) are known to your high content of antioxidants. Objective: To evaluate the effects of soy isoflavones extract (ISO) in the prooxidant/antioxidant balance and morphological changes in the uterus of ovariectomized (OVX) rats. Methods: Twenty 3-month-old adult female Wistar rats were equally divided into four groups: GI- Sham-operated; GII- OVX; GIII- OVX treated with ISO (150mg/kg/day); GIV- OVX treated with estrogen (E2) (10µg/Kg/day). After thirty consecutive days of treatment, the rats were euthanized and the uterus removed. The distal third of the uterine horns were processed for morphometric analyses and immunohistochemistry for protein ki-67. Other uteri regions were kept in liquid nitrogen and subsequently processed for analysis of ROS/RNS quantification (DCF), total antioxidant capacity (TAC) and lipid oxidation status (TBARS). Data were statistically analyzed by one-way ANOVA, complemented by Tukey-Kramer test (p≤0.05). Results: The endometrial and myometrial thickness, as well as the glandular area did not differ significantly between GII and GIII, yet were lower compared to GI and GIV. Since GIV exhibited a higher myometrial thickness than all the other groups. Positivity of nuclei for expression of Ki-67 in both the glandular epithelium and endometrial stroma were significantly higher in GI and GIV compared to GII and GIII. Moreover, ISO treated group showed decreased DCF and TBARS levels compared to GII and also, improvement of TAC compared to GI and GIV. Despite the significant decrease in TBARS, no significant difference in DCF and a decrease in TAC were observed in GIV when compared to GII. Conclusions: Our data show that isoflavones improve antioxidant status and decrease oxidative stress, without promoting trophic effect and cell proliferation in the uterus of rats.
Estrogen deficiency at menopause leads to loss of antioxidant defense, which promotes the increase of reactive oxygen/nitrogen species (ROS/RNS) and favors the oxidative stress. Meanwhile, soy isoflavones (ISO) are known to your high content of antioxidants. Objective: To evaluate the effects of soy isoflavones extract (ISO) in the prooxidant/antioxidant balance and morphological changes in the uterus of ovariectomized (OVX) rats. Methods: Twenty 3-month-old adult female Wistar rats were equally divided into four groups: GI- Sham-operated; GII- OVX; GIII- OVX treated with ISO (150mg/kg/day); GIV- OVX treated with estrogen (E2) (10µg/Kg/day). After thirty consecutive days of treatment, the rats were euthanized and the uterus removed. The distal third of the uterine horns were processed for morphometric analyses and immunohistochemistry for protein ki-67. Other uteri regions were kept in liquid nitrogen and subsequently processed for analysis of ROS/RNS quantification (DCF), total antioxidant capacity (TAC) and lipid oxidation status (TBARS). Data were statistically analyzed by one-way ANOVA, complemented by Tukey-Kramer test (p≤0.05). Results: The endometrial and myometrial thickness, as well as the glandular area did not differ significantly between GII and GIII, yet were lower compared to GI and GIV. Since GIV exhibited a higher myometrial thickness than all the other groups. Positivity of nuclei for expression of Ki-67 in both the glandular epithelium and endometrial stroma were significantly higher in GI and GIV compared to GII and GIII. Moreover, ISO treated group showed decreased DCF and TBARS levels compared to GII and also, improvement of TAC compared to GI and GIV. Despite the significant decrease in TBARS, no significant difference in DCF and a decrease in TAC were observed in GIV when compared to GII. Conclusions: Our data show that isoflavones improve antioxidant status and decrease oxidative stress, without promoting trophic effect and cell proliferation in the uterus of rats.
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Citação
TEIXEIRA, Cristiane de Paula. Atividade antioxidante e alterações morfológicas induzidas pelas isoflavonas da soja no útero de ratas ovariectomizadas.2014. 78 f. Dissertação (Mestrado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2014.