Efeitos da privação e da restrição de sono na integridade da pele
Arquivos
Data
2011
Autores
Kahan, Vanessa 
Orientadores
Andersen, Monica Levy
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
A pele e o maior orgao do corpo humano, sendo composta principalmente por elastina, colageno e glicosaminoglicanos. Toda a sequencia de eventos responsaveis pela manutencao da integridade da pele pode ser afetada por fatores endogenos e exogenos, como por exemplo, o estresse. Diversos trabalhos na literatura evidenciam que o sono desempenha uma funcao restauradora no funcionamento do organismo. Alteracoes nesses mecanismos podem afetar a producao de colageno. Diversos efeitos da restricao de sono cronica em animais sugerem uma ruptura na funcao de barreira da pele e mucosas. Sendo assim, a reducao do tempo de sono parece afetar de varias maneiras a composicao e a integridade de diversos sistemas. Nesse sentido, a presente dissertacao realizou um levantamento bibliografico publicado no Artigo 1: Stress, immunity and skin collagen integrity: evidence from animal models and clinical conditions, em que foram coletadas evidencias de que por acao dos glicocorticoides e de alteracoes no sistema imunologico o colageno da pele pode ser prejudicado pela privacao de sono em sua quantidade e qualidade. Com o objetivo de inserir na comunidade cientifica a possivel relacao entre o sono e a integridade de pele, o Artigo 2 (Can poor sleep affect skin integrity?) foi elaborado e publicado em uma revista especializada em hipoteses. Com a finalidade de validar a linhagem escolhida para o presente projeto (hairless) foi realizado um estudo preliminar, em que essa linhagem foi submetida a privacao de sono por 72 horas, juntamente com outras duas linhagens muito utilizadas para estudos de privacao de sono (C57BL/6J e Swiss) para uma comparacao dos efeitos genotoxicos em diversos orgaos e sangue periferico (Artigo 3: Sleep loss induces differential response related to genotoxicity in multiple organs of three different mice strains). Nossos resultados mostram que a linhagem Swiss se mostra mais sensivel aos danos ao DNA provocados pela privacao de sono, no sangue periferico e no figado, mostrando assim, que a linhagem Hairless estaria apta a ser submetida a privacao de sono. No Artigo 4: Is lack of sleep capable of inducing damage in aged skin? (submetido) foi verificado se as consequencias da privacao de sono sobre a integridade do DNA da pele se somariam aos efeitos deleterios do envelhecimento em camundongos. Femeas da linhagem Hairless com 15 meses de idade foram submetidos 72 horas de privacao de sono ou 15 dias de restricao de sono. O dano ao DNA da pele foi avaliado pela tecnica do ensaio em gel de celula unica, mostrando que a perda de sono nao se sobrepoe ao dano genetico ja existente na pele desses animais idosos. Em conjunto, as evidencias aqui expostas, indicam uma relacao multidirecional entre o sono, sistema imunologico e a integridade da pele, porem, e necessario observar outros parametros alem da fragmentacao do DNA
The skin is the largest organ in the human body, being mainly composed by elastin, collagen and glycosaminoglycans. The sequence of events responsible for maintaining the skin integrity can be affected by endogenous and exogenous factors, such as stress. Several studies in the literature show that sleep plays a key role in restoring the organism functioning. Various effects of lack of sleep in animals suggest a break in the skin barrier function and mucous. Therefore, the reduction of sleep time in many ways seems to affect the composition and integrity of various systems. In this sense, this study conducted a literature review, published in Article 1: Stress, immunity and skin collagen integrity: evidence from animal models and clinical condition. In this article were collected evidences of lack of sleep can change the quality and quantity of collagen in the skin, mediated by the action of glucocorticoids and immune system changes. In order to clarify the scientific community about the possible relationship between sleep and skin integrity, Article 2: Can poor sleep affect skin integrity? was published in Medical Hypotheses. In order to validate the strain used for this dissertation (Hairless mice strain) we conducted a preliminary study, published in Article 3 (Sleep loss induces differential response related to genotoxicity in multiple organs of three different mice strains), in wich the animals was submitted to sleep deprivation protocol with two other strains (C57BL/6J) to a comparison of genotoxic effects in several organs e peripheral blood. Our results show that Swiss strain was more responsive to DNA damage caused by sleep deprivation, in blood and liver. thus, the hairless strain would be able to be subjected to sleep deprivation. In Article 4: Is lack of sleep capable of inducing damage in aged skin? (submitted), we verifyed wheter the consequences of sleep loss in the integrity of skin’s DNA were added to the deleterious effects of aging in mice. Females Hairless mice with 15 months of age were submitted to 72 hours of sleep deprivation or to 15 days of sleep restriction. The DNA damage was evaluated by single cell gel (comet) assay, and shows that lack of sleep do not overwrite the genetics damages already existing in the old skin. Taken together, the evidence presented here indicates a multidirectional relationship between sleep, immune system and skin integrity. However, it is necessary to observe other parameters in addition to DNA fragmentation with single cell gel (comet) assay
The skin is the largest organ in the human body, being mainly composed by elastin, collagen and glycosaminoglycans. The sequence of events responsible for maintaining the skin integrity can be affected by endogenous and exogenous factors, such as stress. Several studies in the literature show that sleep plays a key role in restoring the organism functioning. Various effects of lack of sleep in animals suggest a break in the skin barrier function and mucous. Therefore, the reduction of sleep time in many ways seems to affect the composition and integrity of various systems. In this sense, this study conducted a literature review, published in Article 1: Stress, immunity and skin collagen integrity: evidence from animal models and clinical condition. In this article were collected evidences of lack of sleep can change the quality and quantity of collagen in the skin, mediated by the action of glucocorticoids and immune system changes. In order to clarify the scientific community about the possible relationship between sleep and skin integrity, Article 2: Can poor sleep affect skin integrity? was published in Medical Hypotheses. In order to validate the strain used for this dissertation (Hairless mice strain) we conducted a preliminary study, published in Article 3 (Sleep loss induces differential response related to genotoxicity in multiple organs of three different mice strains), in wich the animals was submitted to sleep deprivation protocol with two other strains (C57BL/6J) to a comparison of genotoxic effects in several organs e peripheral blood. Our results show that Swiss strain was more responsive to DNA damage caused by sleep deprivation, in blood and liver. thus, the hairless strain would be able to be subjected to sleep deprivation. In Article 4: Is lack of sleep capable of inducing damage in aged skin? (submitted), we verifyed wheter the consequences of sleep loss in the integrity of skin’s DNA were added to the deleterious effects of aging in mice. Females Hairless mice with 15 months of age were submitted to 72 hours of sleep deprivation or to 15 days of sleep restriction. The DNA damage was evaluated by single cell gel (comet) assay, and shows that lack of sleep do not overwrite the genetics damages already existing in the old skin. Taken together, the evidence presented here indicates a multidirectional relationship between sleep, immune system and skin integrity. However, it is necessary to observe other parameters in addition to DNA fragmentation with single cell gel (comet) assay
Descrição
Citação
KAHAN, Vanessa. Efeitos da privação e da restrição de sono na integridade da pele. 2011. 115f. Dissertação (Mestrado) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2011.