Uso da glicina na prevenção das lesões da entercolite necrosante neonatal: estudo experimental em ratos
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Data
2006
Autores
Meyer, Karine Furtado [UNIFESP]
Orientadores
Martins, José Luiz [UNIFESP]
Tipo
Tese de doutorado
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ISSN da Revista
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Objetivo: Avaliar o efeito da glicina no intestino e em órgãos à distância num modelo
experimental de enterocolite necrosante neonatal em ratos recém-nascidos.
Métodos: Foram utilizados ratos Wistar recém-nascidos distribuídos em três grupos:
G1: controle, G2: animais submetidos a hipóxia-reoxigenação (H-R), G3: animais
submetidos a hipóxia-reoxigenação após uma infusão intraperitoneal de glicina
5%.Segmentos intestinais foram preparados para análise histológica,
imunohistoquímica com caspase 3 clivada e lamina A clivada, como marcadores para
apoptose e dosagem tecidual de Malondialdeído (MDA). O fígado foi preparado para
avaliação imunohistoquímica e dosagem de malondialdeído tecidual, e os rins foram
preparados para avaliação imunohistoquímica.
Resultados: A dosagem de MDA tecidual mostrou que o grupo de animais submetido a
H-R apresentou valores maiores do que o grupo previamente submetido à injeção
intraperitoneal de glicina (p < 0,05). O índice apoptótico pela avaliação da caspase 3
clivada e lamina A clivada foi maior nos animais submetidos à H-R do que nos animais
que receberam previamente glicina em todos os órgãos examinados, porém nos rins a
avaliação pela lamina A clivada não foi significante, quando da avaliação estatística. A
análise histológica não mostrou diferença estatística entre o grupo H-R e o previamente
submetido à injeção de glicina.
Conclusões: A glicina foi capaz de reduzir os efeitos deletérios da hipóxiareoxigenação,
quando se considerou como critério de avaliação a dosagem de MDA e
os índices de apoptose celular e não influenciou, estatisticamente, as alterações
histológicas.
Objective: To evaluate the effect of glycine on the intestine and in distant organs, in an experimental model of neonatal necrotizing enterocolitis in rats. Methods: Newborn Wistar rats were utilized, distributed into three groups: G1: control, G2: animals subjected to hypoxia-reoxygenation (H-R), G3: animals subjected to hypoxia-reoxygenation after intraperitoneal infusion of 5% glycine. Intestinal segments were prepared for histological analysis, immunohistochemistry using cleaved caspase-3 and cleaved lamina-A as markers for apoptosis, and tissue assaying for malonaldehyde (MDA). The liver and kidneys were prepared for immunohistochemical evaluation and the liver alone for tissue MDA assaying. Results: Tissue MDA assaying showed that the animals subjected to H-R alone presented higher values than did those previously subjected to glycine infusion (p < 0.05). The apoptosis index from evaluation of cleaved caspase-3 and cleaved lamina-A was greater in the animals subjected to H-R alone than in those previously subjected to glycine infusion, in all the organs examined. However, in the kidneys the findings from cleaved lamina-A did not present any statistical difference. The histological analysis did not show any statistical difference between the group with H-R alone and the group previously subjected to glycine infusion. Conclusions: Glycine was capable of reducing the deleterious effects from hypoxiareoxygenation, when the MDA assay and cell apoptosis index were considered as the evaluation criteria.
Objective: To evaluate the effect of glycine on the intestine and in distant organs, in an experimental model of neonatal necrotizing enterocolitis in rats. Methods: Newborn Wistar rats were utilized, distributed into three groups: G1: control, G2: animals subjected to hypoxia-reoxygenation (H-R), G3: animals subjected to hypoxia-reoxygenation after intraperitoneal infusion of 5% glycine. Intestinal segments were prepared for histological analysis, immunohistochemistry using cleaved caspase-3 and cleaved lamina-A as markers for apoptosis, and tissue assaying for malonaldehyde (MDA). The liver and kidneys were prepared for immunohistochemical evaluation and the liver alone for tissue MDA assaying. Results: Tissue MDA assaying showed that the animals subjected to H-R alone presented higher values than did those previously subjected to glycine infusion (p < 0.05). The apoptosis index from evaluation of cleaved caspase-3 and cleaved lamina-A was greater in the animals subjected to H-R alone than in those previously subjected to glycine infusion, in all the organs examined. However, in the kidneys the findings from cleaved lamina-A did not present any statistical difference. The histological analysis did not show any statistical difference between the group with H-R alone and the group previously subjected to glycine infusion. Conclusions: Glycine was capable of reducing the deleterious effects from hypoxiareoxygenation, when the MDA assay and cell apoptosis index were considered as the evaluation criteria.
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Citação
Meyer, Karine Furtado. Uso da glicina na prevenção das lesões da entercolite necrosante neonatal: estudo experimental em ratos. 2006. 82 f. Tese (Doutorado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2006.