Efeito da oxigenação hiperbárica nas lesões imediatas provocadas pela radiação ionizante, no íleo de camundongos
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Data
2005
Autores
Carvalho, Adriana Ivete Cornita de [UNIFESP]
Orientadores
Gomes, Paulo de Oliveira [UNIFESP]
Tipo
Dissertação de mestrado
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OBJETIVO:Avaliar os efeitos da OHB em células caliciformes de íleo de camundongos após
a exposição à radiação ionizante. MÉTODO: Foram utilizados 35 camundongos machos da
linhagem C57BL, randomizados e distribuídos em quatro grupos: Grupo I, Controle (GC): 5
animais não irradiados ,submetidos à observação por 24hs, seguidos de eutanásia e ressecção
de um segmento de 2cm do íleo terminal. Grupo II, Irradiado (GRT): 10 animais submetidos a
uma sessão de radioterapia com dose única de 5Gy e após 24hs de observação foram
submetidos à de eutanásia e ressecção de um segmento de 2cm do íleo terminal. Grupo III,
Irradiado/ Oxigenação hiperbárica (GRT/OHB): 10 animais submetidos a uma sessão de
radioterapia com dose única de 5Gy, após 24hs foram expostos a cinco sessões diárias de 90’
de OHB, foram submetidos à eutanásia no 7°dia após a sessão de radioterapia, seguido de
ressecção de um segmento de 2 cm do íleo terminal. Grupo IV, Irradiado/ Sem Oxigenação
hiperbárica (GRT/SOHB): 10 animais submetidos a uma sessão de radioterapia com dose
única de 5 Gy e no 7° dia após a sessão radioterápica foram submetidos a eutanásia e
ressecção de um segmento de 2 cm do íleo terminal. Foram avaliadas as células
caliciformes.Os testes estatísticos utilizados foram, análise de variância de Kruskal-Wallis e
teste de Mann-Whitney, com p ≤ 0,05 . RESULTADOS: Ao comparar o número de células
encontrados, pode-se observar que o Grupo IV >I >II > III, sendo que não existe diferença
entre o grupo I e III. A média de células caliciformes encontradas nos grupos foram GI 6,21;
GII 5,01; GIII 6,09; GIV 16,06. Os estudos demonstraram que o grupo IV apresentou uma
hiperplasia das células caliciformes (p ≤ 0,05). CONCLUSÃO: A exposição às radiações
ionizantes provoca hiperplasia das células caliciformes. A oxigenação hiperbárica diminui as
alterações encontradas nas células caliciformes do íleo de camundongos submetidos à
radioterapia.
Objective: The aim of the present investigation was to analyze the effect of the radiance in the ileum caliciforms cells of the male mice from the line C57BL. Methods: the mice was randomize and distributed in four groups: Group I, control (GC) : 5 animals no irradiated, under 24 hrs observation they are sacrificed and 2cm from terminal ileum was colleted. Group II, irradiated (GRT): 10 animals irradiated with a 5Gy single dose and after 24hrs observation they was sacrificed and 2cm from terminal ileum was colleted. Group III, irradiated/ hyperbaric oxygenic therapy (GRT/OHB): 10 animals irradiated with a 5Gy single dose, after 24hs they was exposed to 5 sections daily with 90’ each, they was sacrificed after 7 days of the radiotherapy and 2cm from terminal ileum was colleted. Group IV, irradiated/ no hyperbaric oxygenic therapy (GRT/SOHB): 10 animals irradiated with a 5Gy single dose, they was sacrificed after 7 days of the radiotherapy and 2cm from terminal ileum was colleted. The Kruskal-Wallis variance test and Mann-Whitney test was used to analyzed the data observed. Results: When we match the number of cells, could be observed that the group IV>I>II>III, but that was not founded a difference between group I and III. The caliciforms cells media founded in groups were GI 6,21; GII 5,01; GIII 6,09; GIV 16,06. The studs showed that the group IV demonstrated an hyperplasic from caliciforms cells (p<0,05). Conclusions: The exposition to the ionic radiation induce an hyperplasic in the number of caliciforms cells. The hyperbaric oxygen therapy induce a decreased of the morphological disturbance founded in the caliciforms cells from mice terminal ileum exposed to the radiotherapy
Objective: The aim of the present investigation was to analyze the effect of the radiance in the ileum caliciforms cells of the male mice from the line C57BL. Methods: the mice was randomize and distributed in four groups: Group I, control (GC) : 5 animals no irradiated, under 24 hrs observation they are sacrificed and 2cm from terminal ileum was colleted. Group II, irradiated (GRT): 10 animals irradiated with a 5Gy single dose and after 24hrs observation they was sacrificed and 2cm from terminal ileum was colleted. Group III, irradiated/ hyperbaric oxygenic therapy (GRT/OHB): 10 animals irradiated with a 5Gy single dose, after 24hs they was exposed to 5 sections daily with 90’ each, they was sacrificed after 7 days of the radiotherapy and 2cm from terminal ileum was colleted. Group IV, irradiated/ no hyperbaric oxygenic therapy (GRT/SOHB): 10 animals irradiated with a 5Gy single dose, they was sacrificed after 7 days of the radiotherapy and 2cm from terminal ileum was colleted. The Kruskal-Wallis variance test and Mann-Whitney test was used to analyzed the data observed. Results: When we match the number of cells, could be observed that the group IV>I>II>III, but that was not founded a difference between group I and III. The caliciforms cells media founded in groups were GI 6,21; GII 5,01; GIII 6,09; GIV 16,06. The studs showed that the group IV demonstrated an hyperplasic from caliciforms cells (p<0,05). Conclusions: The exposition to the ionic radiation induce an hyperplasic in the number of caliciforms cells. The hyperbaric oxygen therapy induce a decreased of the morphological disturbance founded in the caliciforms cells from mice terminal ileum exposed to the radiotherapy
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Citação
São Paulo: [s.n.], 2005. 43 p.