Avaliação da perfusão sequencial com as soluções de euro-Collins e Belzer para preservação do pâncreas em ratos
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Data
2005
Autores
Schraibman, Vladimir [UNIFESP]
Orientadores
Goldenberg, Alberto [UNIFESP]
Tipo
Dissertação de mestrado
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Objetivo: Investigar o efeito das soluções de Euro-collins e Belzer na perfusão sequencial do pâncreas durante a preservação. Métodos: Foram utilizados 45 ratos Wistar-EPM. Os animais foram distribuídos em 4 grupos, de acordo com a solução utilizada para preservação: Grupo Soro Fisiológico (SF) - pâncreas perfundido e preservado em soro fisiológico; Grupo Euro-Collins (C) pâncreas perfundido e preservado com solução de Euro-Collins; Grupo Belzer (B) pâncreas perfundido e preservado em solução de Belzer e Grupo Euro-Collins/Belzer (CB) - pâncreas perfundido com solução de Euro-Collins e Belzer em partes iguais e seqüencialmente e preservado em solução de Belzer. Após a perfusão, os animais tiveram o pâncreas ressecado e preservado com a solução destinada em 4° C. Dosagens de amilase em 12, 24, 36 e 48 horas foram realizadas no líquido de preservação. Após a última dosagem a peça foi submetida à análise histológica. Foi realizado estudo estatístico a partir dos resultados. Resultados: Os grupos SF e C foram os que apresentaram os maiores valores de amilase ao longo do tempo, sendo superiores e diferentes dos grupos B e CB (p=O,05). Os grupos B e CB apresentaram valores de amilase similares até o período de 24 I horas. A análise histológica demonstrou significância estatística em relação à necrose de ilhotas e edema tecidual. Os grupos B e CB mostraram-se similares I histologicamente (p=O,OO1) e diferentes dos grupos SF e C. Conclusões: A aplicação das soluções de Euro-collins e Belzer de modo seqüencial mostrou-se eficaz na preservação do pâncreas de ratos até o período de 24 horas.
Objective: Investigate the effect of Euro-collins and Belzer solution in the sequential perfusion of the pancreas, during the preservation period. Methods: Forty five Wistar-EPM rats were used. The animals were distributed in 4 groups, according to the solution used during preservation: Saline solution (SF), pancreas perfused and preserved with saline solution; Euro-collins group (C), pancreas perfused and preserved with euro-collins solution; Belzer group (B) pancreas perfused and preserved with belzer solution; Euro-collins/Belzer group (CB), pancreas perfused with euro-collins and belzer solutions in equal parts and sequentially and preserved with Belzer solution. After the perfusion, the animals had their pancreas resected and preserved according to the destinated solution in 4º C. Amilase dosages after 12, 24, 36 e 48 hours were performed in the preservation solution. After last dosage, the pancreas was submitted to histological analysis. Statistical analysis was done. Results: SF and C groups presented the higher amilase levels in the preserving solution during all periods, being superior to C and CB groups (p=0,05). Amilase levels were similar in b and CB groups until 24 hours (p=0,05). Histological analysis was statistically significant for pancreas islet cells and tecidual edema. Groups B and CB were histologically similar (p=0,001) and different from groups SF and C. Conclusions: The use of Euro-collins and Belzer solution sequentially showed to be effective in pancreas preservation in rats until 24 hours.
Objective: Investigate the effect of Euro-collins and Belzer solution in the sequential perfusion of the pancreas, during the preservation period. Methods: Forty five Wistar-EPM rats were used. The animals were distributed in 4 groups, according to the solution used during preservation: Saline solution (SF), pancreas perfused and preserved with saline solution; Euro-collins group (C), pancreas perfused and preserved with euro-collins solution; Belzer group (B) pancreas perfused and preserved with belzer solution; Euro-collins/Belzer group (CB), pancreas perfused with euro-collins and belzer solutions in equal parts and sequentially and preserved with Belzer solution. After the perfusion, the animals had their pancreas resected and preserved according to the destinated solution in 4º C. Amilase dosages after 12, 24, 36 e 48 hours were performed in the preservation solution. After last dosage, the pancreas was submitted to histological analysis. Statistical analysis was done. Results: SF and C groups presented the higher amilase levels in the preserving solution during all periods, being superior to C and CB groups (p=0,05). Amilase levels were similar in b and CB groups until 24 hours (p=0,05). Histological analysis was statistically significant for pancreas islet cells and tecidual edema. Groups B and CB were histologically similar (p=0,001) and different from groups SF and C. Conclusions: The use of Euro-collins and Belzer solution sequentially showed to be effective in pancreas preservation in rats until 24 hours.
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SCHRAIBMAN, Vladimir. Avaliação da perfusão sequencial com as soluções de euro-Collins e Belzer para preservação do pâncreas em ratos. 2005. 64 f.Dissertação (Mestrado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2005.