Estudo morfológico das entero-anastomoses em coelhos com adesivo sintético ou biológico
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Data
2003
Autores
Amaral, Ademir Teixeira do [UNIFESP]
Orientadores
Taha, Murched Omar [UNIFESP]
Tipo
Tese de doutorado
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Resumo
O objetivo foi verificar a eficacia dos adesivos de fibrina e cianoacrilato como coadjuvantes nas anastomoses de intestino delgado de coelhos. Oitenta coelhos, linhagem Nova Zelandia, machos, adultos, foram submetidos a enterectomia de 3cm e enterorrafia e distribuidos em quatro grupos: Grupo A (dois pontos equidistantes); Grupo B (quatro pontos equidistantes); Grupo C (seis pontos equidistantes); Grupo D (oito pontos equidistantes). A anastomose foi realizada com fio de poliglactina 910 e complementada com adesivo sintetico (octilcianoacrilato) ou adesivo biologico (fibrina) circundando toda a periferia da linha anastomotica. No 14° dia de observacao, os animais foram submetidos a uma laparotomia mediana para estudo macroscopico da cavidade abdominal para identificacao e avaliacao de aderencias, deiscencias e/ou fistulas. O segmento intestinal anastomosado foi retirado e feito um molde de alginato para posterior avaliacao do indice de estenose. As pecas retiradas foram processadas para estudo histologico e avaliacao de fibras colagenas, macrofagos, neovascularizacao e granuloma tipo corpo estranho. Nao houve diferenca significante na avaliacao de deiscencia e fistulas com os dois adesivos entre os quatro sub-grupos. A aderencia ocorreu em maior numero nos sub-grupos do adesivo sintetico (p=7.82). O indice de estenose foi maior nos animais do adesivo sintetico somente nos sub-grupos com dois e oito pontos. A analise histologica mostrou que em todos os parametros avaliados (colageno, macrofago, neovascularizacao e granuloma) nao houve diferenca significante entre os dois tipos de adesivos. Concluiu-se que, a avaliacao morfologica das entero-anastomoses usando os adesivos biologico (6brina) e sintetico (cianoacrilato) nao mostrou diferenca significante na sua utilizacao como coadjuvantes de sutura com fio poliglactina 910
The aim of the research was to verify the efficacy of the fibrin or cianoacrylate glue as coadjuvant in the intestinals anastomosis of rabbits. Eighty rabbits, lineage New Zealand, males, adults, were submitted to enterectomy of 3cm and enteroraphy distributed in four groups: Group A (two equidistants stitches); Group B (four equidistants stitches); Group C (six equidistants stitches); Group D (eight equidistants stitches). The anastomosis was made with thread of 910 polyglactina and supplemented with octyl-2-cyanoacrylate adhesive or fibrin glue around of the surface of the anastomosis. In 14 days of observation, the animals were submitted to median laparotomy for macroscopic study of the abdominal cavity for identification and evaluation of adherence, dehiscence and fistula. The segment anastomosed was removed and was maked mould of alginate for later evaluation of the index of stenosis. The parts removed were processed for histological study and evaluation of collagen, macrofages, vessels and granulomas. Significant difference was not presented in the evaluation of dehiscence and fistula with the two adhesives between the four subgroups. The adherence occurred in higher number in the subgroups of the synthetical adhesive (p=7.82). The index of stenosis was higher in the animals of the synthetical adhesive only in the subgroups with two and eight stitches. The histological analysis showed that in all the parameters evaluated (collagen, macrofages, vessels and granulomas) did not present significant difference between the two kinds of adhesives. It was conclued that the morphologic evaluation of the intestinals anastomosis using the synthetical (cyanoacrylate) adhesive or fibrin glue did not show significant difference when used as coadjuvant of suture with thread 910 polyglactyna.
The aim of the research was to verify the efficacy of the fibrin or cianoacrylate glue as coadjuvant in the intestinals anastomosis of rabbits. Eighty rabbits, lineage New Zealand, males, adults, were submitted to enterectomy of 3cm and enteroraphy distributed in four groups: Group A (two equidistants stitches); Group B (four equidistants stitches); Group C (six equidistants stitches); Group D (eight equidistants stitches). The anastomosis was made with thread of 910 polyglactina and supplemented with octyl-2-cyanoacrylate adhesive or fibrin glue around of the surface of the anastomosis. In 14 days of observation, the animals were submitted to median laparotomy for macroscopic study of the abdominal cavity for identification and evaluation of adherence, dehiscence and fistula. The segment anastomosed was removed and was maked mould of alginate for later evaluation of the index of stenosis. The parts removed were processed for histological study and evaluation of collagen, macrofages, vessels and granulomas. Significant difference was not presented in the evaluation of dehiscence and fistula with the two adhesives between the four subgroups. The adherence occurred in higher number in the subgroups of the synthetical adhesive (p=7.82). The index of stenosis was higher in the animals of the synthetical adhesive only in the subgroups with two and eight stitches. The histological analysis showed that in all the parameters evaluated (collagen, macrofages, vessels and granulomas) did not present significant difference between the two kinds of adhesives. It was conclued that the morphologic evaluation of the intestinals anastomosis using the synthetical (cyanoacrylate) adhesive or fibrin glue did not show significant difference when used as coadjuvant of suture with thread 910 polyglactyna.
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Citação
São Paulo: [s.n.], 2003. 61 p.