Comparison of the efficacy of biodegradable and non-biodegradable scintillation liquids on the counting of tritium- and [14C]-labeled compounds

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dc.contributor.author Medeiros, Regina Bitelli [UNIFESP]
dc.contributor.author Godinho, Rosely Oliveira [UNIFESP]
dc.contributor.author Mattos, M.f.s.s. [UNIFESP]
dc.date.accessioned 2015-06-14T13:30:13Z
dc.date.available 2015-06-14T13:30:13Z
dc.date.issued 2003-12-01
dc.identifier http://dx.doi.org/10.1590/S0100-879X2003001200016
dc.identifier.citation Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 12, p. 1733-1739, 2003.
dc.identifier.issn 0100-879X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/1925
dc.description.abstract The widespread use of ³H and 14C in research has generated a large volume of waste mixed with scintillation liquid, requiring an effective control and appropriate storage of liquid radioactive waste. In the present study, we compared the efficacy of three commercially available scintillation liquids, Optiphase HiSafe 3, Ultima-Gold AB (biodegradable) and Insta-Gel-XF (non-biodegradable), in terms of [14C]-glucose and [³H]-thymidine counting efficiency. We also analyzed the effect of the relative amount of water (1.6 to 50%), radioisotope concentration (0.1 to 100 nCi/ml), pH (2 to 10) and color of the solutions (samples containing 0.1 to 1.0 mg/ml of Trypan blue) on the counting efficiency in the presence of these scintillation liquids. There were few significant differences in the efficiency of 14C and ³H counting obtained with biodegradable or non-biodegradable scintillation liquids. However, there was an 83 and 94% reduction in the efficiency of 14C and ³H counting, respectively, in samples colored with 1 mg/ml Trypan blue, but not with 0.1 mg/ml, independent of the scintillation liquid used. Considering the low cost of biodegradable scintillation cocktails and their efficacy, these results show that traditional hazardous scintillation fluids may be replaced with the new safe biodegradable fluids without impairment of ³H and 14C counting efficiency. The use of biodegradable scintillation cocktails minimizes both human and environmental exposure to hazardous solvents. In addition, some biodegradable scintillation liquids can be 40% less expensive than the traditional hazardous cocktails. en
dc.format.extent 1733-1739
dc.language.iso eng
dc.publisher Associação Brasileira de Divulgação Científica
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights Acesso aberto
dc.subject Biodegradable chemicals en
dc.subject Scintillation liquids en
dc.subject Liquid waste en
dc.subject Color quenching en
dc.subject Scintillation counting efficiency en
dc.subject Tritium and 14C counting en
dc.title Comparison of the efficacy of biodegradable and non-biodegradable scintillation liquids on the counting of tritium- and [14C]-labeled compounds en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Unidade de Proteção Radiológica
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Farmacologia
dc.description.affiliationUnifesp UNIFESP, EPM, Unidade de Proteção Radiológica
dc.description.affiliationUnifesp UNIFESP, EPM, Depto. de Farmacologia
dc.identifier.file S0100-879X2003001200016.pdf
dc.identifier.scielo S0100-879X2003001200016
dc.identifier.doi 10.1590/S0100-879X2003001200016
dc.description.source SciELO
dc.identifier.wos WOS:000187175000016



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