Freqüência do pulso da estimulação elétrica nervosa transcutânea na viabilidade do retalho cutâneo randômico isquêmico, em ratos.
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Data
2004
Autores
Liebano, Richard Eloin [UNIFESP]
Orientadores
Ferreira, Lydia Masako [UNIFESP
Tipo
Tese de doutorado
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Objetivo: 0 presente estudo teve como objetivo verificar o efeito da frequencia do pulso da Estimulacao Eletrica Nervosa Transcutanea (TENS) na viabilidade do retalho cutaneo randomico isquemico em ratos. Metodos: 0 material constituiu-se de 45 ratos da linhagem Wistar-EPM. 0 retalho cutaneo randomico foi realizado com dimensoes de 10 x 4 cm e uma barreira plastica foi interposta entre o mesmo e o leito doador. 0 Grupo 1 (Controle) foi anestesiado por uma hora com os eletrodos posicionados na base do retalho sem a administracao do estimulo eletrico, imediatamente apos a operacao e nos 2 dias subsequentes (POI, PO1 e P02). 0 Grupo 2 foi submetido a TENS por 1 hora imediatamente apos a operacao e nos outros dois dias subsequentes com frequencia de 2 Hz e amplitude de 15 mA. 0 Grupo 3 foi submetido a TENS da mesma forma que o grupo 2, porem, com 80 Hz e 15 mA. Resultados: Nos tres grupos foram calculadas as porcentagens de area de necrose (media de 43,11 no Grupo 1, 32,57 no Grupo 2 e 23,52 no Grupo 3) no setimo dia pos-operatorio. Conclusoes: A Estimulacao Eletrica Nervosa Transcutanea foi eficiente no aumento da viabilidade do retalho cutaneo randomico isquemico. Nao houve diferenca entre as 2 frequencias de pulso utilizadas (Grupos 2 e 3)
The aim of this study was to determine the role of Transcutaneous Electrical Nerve Stimulation (TENS) in viability of random skin flap. The material of this study was 45 Wistar-EPM rats. The random skin flap measured 10 x 4 cm and a plastic barrier was interposed between the flap and donnor site bed. Group 1 (control) was anesthetized during one hour with the electrodes positioned in the base of the flap without the administration of the electric stimulus immediately after the surgery and in the other two subsequent days. Group 2 was submitted to TENS during 1 hour immediately after the surgery and in the other two subsequent days with frequency of 2 Hz and amplitude of 15 mA. Group 3 was submitted to TENS during 1 hour immediately after the surgery and in the other two subsequent days too, but with frequency of 80 Hz and amplitude of 15 mA. The percentage of necrotic area (average of 43,11 in Group 1, 32,57 in Group 2, and 23,52 in Group 3) was calculated in the seventh postoperative day in all groups.Statistical analysis proved that TENS was efficient in increase of random skin flap viability compared with control group and there was no difference between groups 2 and 3.
The aim of this study was to determine the role of Transcutaneous Electrical Nerve Stimulation (TENS) in viability of random skin flap. The material of this study was 45 Wistar-EPM rats. The random skin flap measured 10 x 4 cm and a plastic barrier was interposed between the flap and donnor site bed. Group 1 (control) was anesthetized during one hour with the electrodes positioned in the base of the flap without the administration of the electric stimulus immediately after the surgery and in the other two subsequent days. Group 2 was submitted to TENS during 1 hour immediately after the surgery and in the other two subsequent days with frequency of 2 Hz and amplitude of 15 mA. Group 3 was submitted to TENS during 1 hour immediately after the surgery and in the other two subsequent days too, but with frequency of 80 Hz and amplitude of 15 mA. The percentage of necrotic area (average of 43,11 in Group 1, 32,57 in Group 2, and 23,52 in Group 3) was calculated in the seventh postoperative day in all groups.Statistical analysis proved that TENS was efficient in increase of random skin flap viability compared with control group and there was no difference between groups 2 and 3.
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Citação
LIEBANO, Richard Eloin. Estimulação Elétrica Nervosa Transcutânea na viabilidade do retalho cutâneo randômico isquêmico em ratos. 2004. 62 f. Tese (Doutorado) – Escola Paulista de Medicina, Universidade Federal de São Paulo. São Paulo, 2004