Decorin is one of the proteoglycans expressed in Walker 256 rat mammary carcinoma

Decorin is one of the proteoglycans expressed in Walker 256 rat mammary carcinoma

Author Oba-Shinjo, Sueli Mieko Autor UNIFESP Google Scholar
Berto, Alessandra Gutierrez Andrade Autor UNIFESP Google Scholar
Passerotti, Carlo Camargo Autor UNIFESP Google Scholar
Barbosa, C.d. Autor UNIFESP Google Scholar
Sampaio, Lucia de Oliveira Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Proteoglycan and glycosaminoglycan content was analyzed in a model of rat mammary carcinoma to study the roles of these compounds in tumorigenesis. Hyaluronic acid and proteoglycans bearing chondroitin and/or dermatan sulfate chains were detected in solid tumors obtained after subcutaneous inoculation of Walker 256 rat carcinoma cells. About 10% of sulfated glycosaminoglycan chains corresponded to heparan sulfate. The small leucine-rich proteoglycan, decorin, was identified as one of the proteoglycans, in addition to others of higher molecular weight, by cross-reaction with an antiserum raised against pig laryngeal decorin and by N-terminal amino acid sequencing. Decorin was separated from other proteoglycans by hydrophobic chromatography and its complete structure was determined. It has a molecular weight of about 85 kDa and a dermatan chain of 45 kDa with 4-sulfated disaccharides. After degradation of the glycosaminoglycan chain, three core proteins of different molecular weight (36, 46 and 56 kDa) were identified. The presence of hyaluronic acid and decorin has been reported in a variety of tumors and tumor cells. In the Walker 256 mammary carcinoma model, hyaluronic acid may play an important role in tumor progression, since it provides a more hydrated extracellular matrix. On the other hand, decorin, which is expressed by stromal cells, represents a host defense response to tumor growth.
Keywords Proteoglycan
Walker 256 tumor
Language English
Date 2003-08-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 8, p. 1079-1089, 2003.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 1079-1089
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000185012900016
SciELO ID S0100-879X2003000800015 (statistics in SciELO)

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