The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission

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dc.contributor.author Borja-oliveira, C.r.
dc.contributor.author Durigon, A.m.
dc.contributor.author Vallin, A.c.c. [UNIFESP]
dc.contributor.author Toyama, M.h.
dc.contributor.author Souccar, Caden [UNIFESP]
dc.contributor.author Marangoni, S.
dc.contributor.author Rodrigues-simioni, L.
dc.date.accessioned 2015-06-14T13:30:00Z
dc.date.available 2015-06-14T13:30:00Z
dc.date.issued 2003-05-01
dc.identifier http://dx.doi.org/10.1590/S0100-879X2003000500009
dc.identifier.citation Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 617-624, 2003.
dc.identifier.issn 0100-879X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/1708
dc.description.abstract The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml) produced only partial neuromuscular blockade (30.7 ± 8.0%, N = 3) after 120 min and the initial inhibition and the secondary increase of the twitch responses caused by the venom were prolonged and pronounced and the response to KCl was unchanged. These results indicate that B.n. pauloensis venom is neurotoxic, acting primarily at presynaptic sites, and that enzyme activity may be involved in this pharmacological action. en
dc.format.extent 617-624
dc.language.iso eng
dc.publisher Associação Brasileira de Divulgação Científica
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights Acesso aberto
dc.subject Chick biventer cervicis en
dc.subject Myotoxicity en
dc.subject Neurotoxicity en
dc.subject Phospholipase A2 en
dc.subject Presynaptic action en
dc.title The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission en
dc.type Artigo
dc.contributor.institution Universidade Estadual de Campinas (UNICAMP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Estadual de Campinas Faculdade de Ciências Médicas Departamento de Farmacologia
dc.description.affiliation Universidade Estadual de Campinas Instituto de Biologia Departamento de Bioquímica
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Farmacologia
dc.description.affiliationUnifesp UNIFESP, EPM, Depto. de Farmacologia
dc.identifier.file S0100-879X2003000500009.pdf
dc.identifier.scielo S0100-879X2003000500009
dc.identifier.doi 10.1590/S0100-879X2003000500009
dc.description.source SciELO
dc.identifier.wos WOS:000183100000009



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