The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission

The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission

Author Borja-oliveira, C.r. Google Scholar
Durigon, A.m. Google Scholar
Vallin, A.c.c. Autor UNIFESP Google Scholar
Toyama, M.h. Google Scholar
Souccar, Caden Autor UNIFESP Google Scholar
Marangoni, S. Google Scholar
Rodrigues-simioni, L. Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Abstract The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml) produced only partial neuromuscular blockade (30.7 ± 8.0%, N = 3) after 120 min and the initial inhibition and the secondary increase of the twitch responses caused by the venom were prolonged and pronounced and the response to KCl was unchanged. These results indicate that B.n. pauloensis venom is neurotoxic, acting primarily at presynaptic sites, and that enzyme activity may be involved in this pharmacological action.
Keywords Chick biventer cervicis
Myotoxicity
Neurotoxicity
Phospholipase A2
Presynaptic action
Language English
Date 2003-05-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 617-624, 2003.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 617-624
Origin http://dx.doi.org/10.1590/S0100-879X2003000500009
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000183100000009
SciELO ID S0100-879X2003000500009 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/1708

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