ZapA, a possible virulence factor from Proteus mirabilis exhibits broad protease substrate specificity

ZapA, a possible virulence factor from Proteus mirabilis exhibits broad protease substrate specificity

Author Anéas, M.a.f. Google Scholar
Portaro, Fernanda Calheta Vieira Autor UNIFESP Google Scholar
Lebrun, Ivo Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Palma, M.s. Google Scholar
Fernandes, B.l. Google Scholar
Institution Universidade de São Paulo (USP)
Instituto Butantan CEPID-FAPESP Centro de Toxinologia Aplicada
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (UNESP)
Abstract The opportunistic bacterium Proteus mirabilis secretes a metalloprotease, ZapA, considered to be one of its virulence factors due to its IgA-degrading activity. However, the substrate specificity of this enzyme has not yet been fully characterized. In the present study we used fluorescent peptides derived from bioactive peptides and the oxidized ß-chain of insulin to determine the enzyme specificity. The bradykinin- and dynorphin-derived peptides were cleaved at the single bonds Phe-Ser and Phe-Leu, with catalytic efficiencies of 291 and 13 mM/s, respectively. Besides confirming already published cleavage sites, a novel cleavage site was determined for the ß-chain of insulin (Val-Asn). Both the natural and the recombinant enzyme displayed the same broad specificity, demonstrated by the presence of hydrophobic, hydrophilic, charged and uncharged amino acid residues at the scissile bonds. Native IgA, however, was resistant to hydrolysis by ZapA.
Keywords Proteus mirabilis
substrate specificity
fluorogenic peptides
insulin ß-chain
Language English
Date 2001-11-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 11, p. 1397-1403, 2001.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 1397-1403
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000172765400004
SciELO ID S0100-879X2001001100004 (statistics in SciELO)

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