Aspectos morfológicos e morfométricos da reparação tecidual de feridas cutâneas de ratos com e sem tratamento com solução de papaína a 2%
Arquivos
Data
1991
Autores
Sanchez Neto, Rafael 
Orientadores
Barone, Boris
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Neste trabalho tivemos o objetivo de estudar a reparação tecidual de feridas cutâneas de ratos, com tratamento de solução de papaina a 2%. Foram utilizados 40 ratos, adultos, da linhagem Wistar. Em cada animal foi realizado uma ferida de 2,0cm de diâmetro na sua região dorsal. Os animais foram divididos em dois grupos de 20 ratos, sendo Grupo Controle e Grupo Papaina. Cada grupo foi subdividido em quatro subgrupos de cinco animais, de acordo com a fase de cicatrização a ser analisada ou seja no 3°, 70°, 14° e 21° dia de pós-operatório. Nas respectivas datas, os animais foram sacrificados, sendo as feridas retiradas e encaminhadas para exame histológico. Realizou-se um estudo comparativo entre os dois grupos de ferida, através da análise morfológica e histométrica, para contagem de leucócitos, fibroblastos e fibras colágenas. Os dados obtidos da histometria foram submetidos à análise estatística pelos testes de Mann-Whitney e Kruskal-Wallis. Os resultados mostraram que no grupo tratado com soluço de papaina houve uma epitelização mais rápida do que no grupo controle, que o número de leucócitos foi sempre menor no grupo tratado com solução de papaina, o número de fibroblastos foi maior no grupo papaina do que no grupo controle e o número de fibras colágenas do grupo tratado com papaina foi sempre superior ao do grupo controle. Portanto, concluimos que a reparação tecidual nas feridas tratadas com papaina evoluiu de forma mais rápida que no grupo controle
The present paper has as its main goal the study of the tissue repair of cutaneous wound in rats by the treatment with papain solution at 2%. To perform the research it were used 40 Wistar adult male rats. In each rat it was made a 2.0cm wound on its dorsal region. The animals were divided in two groups of 20 rats each, called Control Group and Papain Group. Each one of these groups were divided again in 4 groups with 5 animals each, according to the cicatrization phase to be analised, that meant the third, seventh fourteenth and twenty-first post operative days. At the respective dates the animals were killed and had their wounds removed and sent to histological exam. It was performed a comparative study between the two wound groups through morphological and histometrical analysis in order to count leucocyts, fiberblasts and colagen fibers. the resultant data from histometry were submitted to statistical analysis by Mann-Whitney and Kruskal-Wallis tests. The results showed that in the papain group, there was a faster epitelial growth than the Control Group. In the same way, the number of leucocyts was always smaller in the papain group, the fiberblasts number was bigger in the papain group and the number of colagenous fibers in the papain group, as well always bigger in relation to the control group. It can be conclued that the tissue repair at the wounds treated with papain had a faster evolution than the control group.
The present paper has as its main goal the study of the tissue repair of cutaneous wound in rats by the treatment with papain solution at 2%. To perform the research it were used 40 Wistar adult male rats. In each rat it was made a 2.0cm wound on its dorsal region. The animals were divided in two groups of 20 rats each, called Control Group and Papain Group. Each one of these groups were divided again in 4 groups with 5 animals each, according to the cicatrization phase to be analised, that meant the third, seventh fourteenth and twenty-first post operative days. At the respective dates the animals were killed and had their wounds removed and sent to histological exam. It was performed a comparative study between the two wound groups through morphological and histometrical analysis in order to count leucocyts, fiberblasts and colagen fibers. the resultant data from histometry were submitted to statistical analysis by Mann-Whitney and Kruskal-Wallis tests. The results showed that in the papain group, there was a faster epitelial growth than the Control Group. In the same way, the number of leucocyts was always smaller in the papain group, the fiberblasts number was bigger in the papain group and the number of colagenous fibers in the papain group, as well always bigger in relation to the control group. It can be conclued that the tissue repair at the wounds treated with papain had a faster evolution than the control group.
Descrição
Citação
SANCHEZ NETO, Rafael. Aspectos morfológicos e morfométricos da reparação tecidual de feridas cutâneas de ratos com e sem tratamento com solução de papaína a 2%. Dissertação (Mestrado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 1991.