Now showing items 1-10 of 10

    • Comparative time-course study of aminoacyl- and dipeptidyl-resin hydrolysis 

      Jubilut, Guita Nicolaewsky [UNIFESP]; Marchetto, Reinaldo; Cilli, Eduardo Maffud [UNIFESP]; Oliveira, Eliandre [UNIFESP]; Miranda, Antonio [UNIFESP]; Tominaga, Mineko [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Sociedade Brasileira de Química, 1997-01-01)
      The classic hydrolysis procedure for quantification of resin-bound aminoacyl and peptidyl groups with 12 N HCl: propionic acid was reevaluated by studying the influence of the nature of the resin and the resin-bound group. ...

    • First purification of heparan sulfate disaccharides with an amine resin used as solid support for peptide synthesis 

      Carvalho, RSH; Tersariol, ILS; Nader, H. B.; Nakaie, C. R. (Elsevier B.V., 2000-01-03)
      For the first time, highly substituted benzhydryramine-resin (BHAR), a solid support used only for the peptide synthesis method was assayed successfully as an anion exchanger resin for fractionation of (-2)-, (-3)- and ...

    • Importance of the solvation degree of peptide-resin beads for amine groups determination by the picric acid method 

      Cilli, Eduardo Maffud [UNIFESP]; Jubilut, Guita Nicolaewsky [UNIFESP]; Ribeiro, Suely C. F. [UNIFESP]; Oliveira, Eliandre [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Sociedade Brasileira de Química, 2000-10-01)
      The classic and important picric acid method used in polymers biochemical and chemical fields of polymers for amine group quantification was chosen in this work as a model for evaluating the influence of the resin bead ...

    • Novel Copoly(Styrene-Divinylbenzene)-Resins with Different Phenylmethylamine Groups for Use in Peptide Synthesis Method 

      Souza, Sinval Estevam Gregorio de [UNIFESP]; Malavolta, Luciana [UNIFESP]; Cilli, Eduardo Maffud [UNIFESP]; Schreier, Shirley [UNIFESP]; Jubilut, Guita Nicolaewsky [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Bentham Science Publ Ltd, 2015-01-01)
      Differently than the 4-methylbenzhydrylamine-resin (MBHAR) which contains a methyl group coupled to the phenylmethylamine-functionalized copoly(styrene-divinilbenzene) structure, alternative resins containing the ...

    • Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography 

      Carvalho, RSH; Ianzer, D. A.; Malavolta, L.; Rodrigues, M. M.; Cilli, E. M.; Nakaie, C. R. (Elsevier B.V., 2005-03-25)
      This paper deals with an unusual application for a copolymer of styrene-1 % divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. the so-called aminomethyl resin (AMR), ...

    • Solvation of polymers as model for solvent effect investigation: proposition of a novel polarity scale 

      Malavolta, L.; Oliveira, E.; Cilli, E. M.; Nakaie, C. R. (Elsevier B.V., 2002-05-27)
      A precise understanding of the polymer solvation effect has been considered crucial to many modem methods, but its dependence on the polarity of the medium is still not entirely established. To more thoroughly address this ...

    • Study of the effect of the peptide loading and solvent system in SPPS by HRMAS-NMR 

      Valente, A. P.; Almeida, FCL; Nakaie, C. R.; Schreier, S.; Crusca, E.; Cilli, E. M. (Wiley-Blackwell, 2005-09-01)
      The SPPS methodology has continuously been investigated as a valuable model to monitor the solvation properties of polymeric materials. in this connection, the present work applied HRMAS-NMR spectroscopy to examine the ...

    • Use of commercial anion-exchange resins as solid support for peptide synthesis and affinity chromatography 

      Nakaie, C. R.; Lanzer, D. A.; Malavolta, L.; Cilli, E. M.; Rodrigues, M. M. (Elsevier B.V., 2003-07-01)
      This report demonstrates that due to the presence of residual reactive sites in their matrices, classical diethylaminoethyl-attaching commercial anion-exchanger resins such as DEAE-MacroPrep and DEAE-Sephadex A50 supports ...

    • Use of the same polymer for synthesis and purification of peptides 

      Silva, Elias Horacio da [UNIFESP]; Etchegaray, Augusto [UNIFESP]; Carvalho, Regina S. H. [UNIFESP]; Jubilut, Guita Nicolaewsky [UNIFESP]; Miranda, Antonio [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Sociedade Brasileira de Química, 2005-04-01)
      This work reveals an uncommon but valuable biotechnological approach regarding the use of a same polymer (benzhydrylamine-resin, BHAR) for synthesis and anion exchange purification of peptides. Initially, the octapeptide ...

    • Uso da FTIR na obtenção de resinas e na síntese de peptídeos em fase sólida 

      Cespedes, Graziely Ferreira; Vicente, Eduardo Festozo; Cilli, Eduardo Maffud [UNIFESP]; Jubilut, Guita Nicolaewsky [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Sociedade Brasileira de Química, 2011-01-01)
      Despite the increase in peptide chain aggregation, which decreases the rate of coupling reactions, the synthesis and use of very highly substituted resins still remains as a controversial point in the SPPS, due to its clear ...