Now showing items 1-10 of 11

    • Effect of aqueous and nonaqueous fixatives on the quantitative estimation of collagen-proteoglycan interaction in tissue sections 

      Toledo, OMS; Taniwaki, N. N.; Saldiva, PHN; Montes, G. S. (Williams & Wilkins, 1996-05-01)
      The present study was designed to assess the influence of aqueous and nonaqueous fixatives on the quantitative estimation of collagen-proteoglycan interaction in tissue sections. Tissues containing different collagen types ...

    • Expression of Heparanase Isoforms in Intervertebral Discs Classified According to Pfirrmann Grading System for Disc Degeneration 

      Reis Rodrigues, Luciano Miller [UNIFESP]; Oliveira, Lilian Zerbinatti de [UNIFESP]; Silva Pinhal, Maria Aparecida da [UNIFESP] (Lippincott Williams & Wilkins, 2013-06-01)
      Study Design. This is a quantitative study of heparanase isoforms expression in degenerative and nondegenerative intervertebral discs (IVDs).Objective. To quantify the expression of both heparanase isoforms (HPSE1 and ...

    • Gene expression of extracellular matrix proteoglycans in human cyclosporin-induced gingival overgrowth 

      Gnoatto, Nelson; Lotufo, Roberto FM; Toffoletto, Odaly [UNIFESP]; Marquezini, Monica Valeria [UNIFESP] (Amer Acad Periodontology, 2003-12-01)
      Background: Gingival overgrowth is one of the side effects associated with the systemic use of cyclosporin A (CsA). in vitro studies on the extracellular matrix of gingival tissues have demonstrated an altered composition, ...

    • Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase 

      Franco, Celia Regina Cavichlolo [UNIFESP]; Rocha, Hugo Alexandre Oliveira [UNIFESP]; Trindade, Edvaldo da Silva [UNIFESP]; Santos, Isabel Anunciacao Neves dos [UNIFESP]; Leite, Edda Lisboa [UNIFESP]; Veiga, Silvio Sanches [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Dietrich, Carl Peter [UNIFESP] (Associação Brasileira de Divulgação Científica, 2001-08-01)
      We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient ...

    • Heparan sulfate proteoglycans mediate the invasion of cardiomyocytes by Trypanosoma cruzi 

      Calvet, C. M.; Toma, L.; De Souza, F. R.; De Meirelles, M. D.; Pereira, MCS (Soc Protozoologists, 2003-03-01)
      Cytoadherence is an important step for the invasion of a mammalian host cell by Trypanosoma cruzi. Cell surface macromolecules are implicated in the T. cruzi-cardiomyocyte recognition process. Therefore, we investigated ...

    • Heparanase-2, syndecan-1, and extracellular matrix remodeling in colorectal carcinoma 

      Peretti, Thais [UNIFESP]; Waisberg, Jaques [UNIFESP]; Mader, Ana Maria Amaral Antonio [UNIFESP]; Matos, Leandro Luongo de [UNIFESP]; Costa, Ricardo B. da; Conceição, Gleice Margarete de Souza [UNIFESP]; Lopes, Antonio Carlos [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Pinhal, Maria Aparecida da Silva [UNIFESP] (Lippincott Williams & Wilkins, 2008-08-01)
      Aim To propose a quantitative method to detect heparanase-2 (HPA2) and syndecan-1 (Syn-1) using immunohistochemistry in colorectal (colon and rectal) carcinomas compared with nonneoplastic tissues and evaluate the possible ...

    • Inhibition of heparin synthesis by methotrexate in rats in vivo 

      Marcondes, S.; Bau, E. C.; Antunes, E.; Dietrich, C. P.; Nader, H. B.; De Nucci, G. (Elsevier B.V., 2002-07-15)
      The content and synthesis of heparin and mast cell-dependent skin oedema (as an indirect evaluation of histamine and serotonin content) were investigated in the rat skin after chronic treatment with compound 48/80, a mast ...

    • Melanocyte transformation associated with substrate adhesion impediment 

      Oba-Shinjo, Sueli Mieko [UNIFESP]; Correa, Mariangela [UNIFESP]; Ricca, Tatiana Iervolino [UNIFESP]; Molognoni, Fernanda [UNIFESP]; Pinhal, Maria Aparecida da Silva [UNIFESP]; Neves, Izabel A. [UNIFESP]; Marie, Sueli Kazue Nagahashi; Sampaio, Lucia de Oliveira [UNIFESP]; Nader, Helena Bonciani [UNIFESP]; Chammas, Roger; Jasiulionis, Miriam Galvonas [UNIFESP] (Neoplasia Press, 2006-03-01)
      Exclude experimental models of malignant transformationemploy chemical and physical carcinogens or genetic manipulations to study tumor progression. in this work, different melanoma cell lines were established after ...

    • Modulation of the Plasma Kallikrein-Kinin System Proteins Performed by Heparan Sulfate Proteoglycans 

      Motta, Guacyara [UNIFESP]; Tersariol, Ivarne L. S. [UNIFESP] (Frontiers Media Sa, 2017)
      Human plasma kallikrein-kinin system proteins are related to inflammation through bradykinin. In the proximity of its target cells, high molecular weight kininogen (H-kininogen) is the substrate of plasma kallikrein, which ...

    • Ultrastructural and immunohistochemical analysis of proteoglycans in mouse pubic symphysis 

      Pinheiro, M. C.; Joazeiro, P. P.; Mora, O. A.; Toledo, OMS (Elsevier B.V., 2003-01-01)
      Proteoglycans were accurately localized in mouse pubic symphyseal tissues using the cuprolinic blue method. Specific glycosaminoglycans degradative enzymes, together with chondroitin sulfate and decorin antibodies, allowed ...