Now showing items 1-9 of 9

    • Assessment of the Aggregation Propensity of the beta-amyloid Peptide During the Synthesis and when Free in Solution 

      Malavolta, Luciana [UNIFESP]; Pinto, Marcelo Rodrigo Silva [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Bentham Science Publ Ltd, 2013-08-01)
      This work developed an alternative approach targeting the evaluation of the aggregation propensity of the (1-42) beta-amyloid peptide (Alzheimer's disease) and some segments, either attached to a polymer during their ...

    • Direct electron paramagnetic resonance monitoring of the peptide synthesis coupling reaction in polymeric support 

      Nakaie, Clovis R.; Malavolta, Luciana; Schreier, Shirley; Trovatti, Eliane; Marchetto, Reinaldo (Elsevier B.V., 2006-06-14)
      This work demonstrates, for the first time. a time-resolved electron paramagnetic resonance (EPR) monitoring of a chemical reaction occurring in a polymeric structure. the progress of the coupling of a N-alpha-tert-butyl ...

    • Importance of the solvation degree of peptide-resin beads for amine groups determination by the picric acid method 

      Cilli, Eduardo Maffud [UNIFESP]; Jubilut, Guita Nicolaewsky [UNIFESP]; Ribeiro, Suely C. F. [UNIFESP]; Oliveira, Eliandre [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Sociedade Brasileira de Química, 2000-10-01)
      The classic and important picric acid method used in polymers biochemical and chemical fields of polymers for amine group quantification was chosen in this work as a model for evaluating the influence of the resin bead ...

    • Novel Copoly(Styrene-Divinylbenzene)-Resins with Different Phenylmethylamine Groups for Use in Peptide Synthesis Method 

      Souza, Sinval Estevam Gregorio de [UNIFESP]; Malavolta, Luciana [UNIFESP]; Cilli, Eduardo Maffud [UNIFESP]; Schreier, Shirley [UNIFESP]; Jubilut, Guita Nicolaewsky [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Bentham Science Publ Ltd, 2015-01-01)
      Differently than the 4-methylbenzhydrylamine-resin (MBHAR) which contains a methyl group coupled to the phenylmethylamine-functionalized copoly(styrene-divinilbenzene) structure, alternative resins containing the ...

    • Peptide dissociation in solution or bound to a polymer: comparative solvent effect 

      Malavolta, L.; Nakaie, C. R. (Elsevier B.V., 2004-10-11)
      Dissociation of peptide when in solution or attached to a polymer was investigated. Magnified solvation of peptide-resins occurred in solvent with similar polarity. Conversely the solubilization of peptides was not usually ...

    • Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography 

      Carvalho, RSH; Ianzer, D. A.; Malavolta, L.; Rodrigues, M. M.; Cilli, E. M.; Nakaie, C. R. (Elsevier B.V., 2005-03-25)
      This paper deals with an unusual application for a copolymer of styrene-1 % divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. the so-called aminomethyl resin (AMR), ...

    • Solvation of polymers as model for solvent effect investigation: proposition of a novel polarity scale 

      Malavolta, L.; Oliveira, E.; Cilli, E. M.; Nakaie, C. R. (Elsevier B.V., 2002-05-27)
      A precise understanding of the polymer solvation effect has been considered crucial to many modem methods, but its dependence on the polarity of the medium is still not entirely established. To more thoroughly address this ...

    • Use of commercial anion-exchange resins as solid support for peptide synthesis and affinity chromatography 

      Nakaie, C. R.; Lanzer, D. A.; Malavolta, L.; Cilli, E. M.; Rodrigues, M. M. (Elsevier B.V., 2003-07-01)
      This report demonstrates that due to the presence of residual reactive sites in their matrices, classical diethylaminoethyl-attaching commercial anion-exchanger resins such as DEAE-MacroPrep and DEAE-Sephadex A50 supports ...

    • Use of the same polymer for synthesis and purification of peptides 

      Silva, Elias Horacio da [UNIFESP]; Etchegaray, Augusto [UNIFESP]; Carvalho, Regina S. H. [UNIFESP]; Jubilut, Guita Nicolaewsky [UNIFESP]; Miranda, Antonio [UNIFESP]; Nakaie, Clovis Ryuichi [UNIFESP] (Sociedade Brasileira de Química, 2005-04-01)
      This work reveals an uncommon but valuable biotechnological approach regarding the use of a same polymer (benzhydrylamine-resin, BHAR) for synthesis and anion exchange purification of peptides. Initially, the octapeptide ...