Now showing items 1-10 of 13

    • Cathepsin X binds to cell surface heparan sulfate proteoglycans 

      Nascimento, F. D.; Rizzi, CCA; Nantes, I. L.; Stefe, L.; Turk, B.; Carmona, A. K.; Nader, H. B.; Juliano, L.; Tersariol, ILS (Elsevier B.V., 2005-04-15)
      Glycosaminoglycans have been shown to be important regulators of activity of several papain-like cathepsins. Binding of glycosaminoglycans to cathepsins thus directly affects catalytic activity, stability or the rate of ...

    • Colocalization of heparin and histamine in the intracellular granules of test cells from the invertebrate Styela plicata (Chordata-Tunicata) 

      Cavalcante, MCM; Andrade, L. R. de; Santos-Pinto, C. D.; Straus, A. H.; Takahashi, H. K.; Allodi, S.; Pavao, MSG (Elsevier B.V., 2002-03-01)
      In most ascidian species the oocytes are surrounded by two types of accessory cells called follicle cells and test cells. Test cells are located on the periphery of oocytes and remain in the perivitelline space during egg ...

    • CrataBL, a lectin and Factor Xa inhibitor, plays a role in blood coagulation and impairs thrombus formation 

      Salu, Bruno R. [UNIFESP]; Ferreira, Rodrigo S. [UNIFESP]; Brito, Marlon V. [UNIFESP]; Ottaiano, Tatiana F. [UNIFESP]; Cruz, Jose Walber M. C. [UNIFESP]; Silva, Mariana Cristina Cabral [UNIFESP]; Correia, Maria Tereza S.; Paiva, Patricia M. G.; Maffei, Francisco Humberto de Abreu; Oliva, Maria Luiza Vilela [UNIFESP] (Walter de Gruyter Gmbh, 2014-09-01)
      Arterial thrombosis is an important complication of diabetes and cancer, being an important target for therapeutic intervention. Crataeva tapia bark lectin (CrataBL) has been previously shown to have hypoglycemiant effect ...

    • Distribution of sulfated glycosaminoglycans in the animal kingdom: widespread occurrence of heparin-like compounds in invertebrates 

      Medeiros, G. F.; Mendes, A.; Castro, RAB; Bau, E. C.; Nader, H. B.; Dietrich, C. P. (Elsevier B.V., 2000-07-26)
      Sulfated glycosaminoglycans were isolated from 23 species of 13 phyla of invertebrates and characterized by their electrophoretic migration in three different buffer systems coupled with enzymatic degradation using bacterial ...

    • Heparin binding proteins from Leishmania (Viannia) braziliensis promastigotes 

      Azevedo-Pereira, R. L.; Pereira, M. C. S.; Oliveria Junior, F. O. R.; Brazil, R. P.; Cortes, L. M. C.; Madeira, M. F.; Santos, A. L. S.; Toma, L.; Alves, C. R. (Elsevier B.V., 2007-04-30)
      We have examined the heparin binding proteins from Leishmania (Viannia) braziliensis promastigotes (HBP-Lb) by chromatography assays. the proposed strategy to isolate an enriched fraction of the HBP-Lb consisted of an ...

    • Heparin Induces Rat Aorta Relaxation via Integrin-Dependent Activation of Muscarinic M-3 Receptors 

      Paredes-Gamero, Edgar Julian [UNIFESP]; Medeiros, Valquiria Pereira de [UNIFESP]; Farias, Eduardo Henrique Cunha de [UNIFESP]; Justo, Giselle Zenker [UNIFESP]; Trindade, Edvaldo da Silva [UNIFESP]; Andrade-Lopes, Ana L.; Godinho, Rosely Oliveira [UNIFESP]; Miranda, Antonio [UNIFESP]; Ferreira, Alice Teixeira [UNIFESP]; Tersariol, Ivarne Luis dos Santos [UNIFESP]; Nader, Helena Bonciani [UNIFESP] (Lippincott Williams & Wilkins, 2010-10-01)
      Previous reports have shown that heparin may promote human hypotension and vascular relaxation by elevation of NO levels through unclear mechanisms. We hypothesized that endothelial muscarinic M-3 receptor activation ...

    • Heparins and heparinoids: Occurrence, structure and mechanism of antithrombotic and hemorrhagic activities 

      Nader, Helena Bonciani [UNIFESP]; Lopes, Carla C.; Rocha, Hugo Alexande de Oliveira [UNIFESP]; Santos, Elizeu Antunes dos [UNIFESP]; Dietrich, Carl Peter [UNIFESP] (Bentham Science Publ Ltd, 2004-01-01)
      The correlation between structure, anticloting, antithrombotic and hemorrhagic activities of heparin, heparan sulfate, low molecular weight heparins and heparin-like compounds from various sources that are in used in ...

    • Immunocytochemical localization of heparin in secretory granules of rat peritoneal mast cells using a monoclonal anti-heparin antibody (ST-1) 

      Oliani, Sonia M.; Freymuller, Edna [UNIFESP]; Takahashi, Helio K. [UNIFESP]; Straus, Anita H. [UNIFESP] (Histochemical Soc Inc, 1997-02-01)
      We performed immunogold labeling with an ST-1 monoclonal antibody (IgM), specific for intact heparin, to define the subcellular localization of heparin in mast cells. Rat peritoneal mast cells were fixed by a modified ...

    • Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood 

      Brunialti, Milena Karina Coló [UNIFESP]; Kallas, Esper Georges [UNIFESP]; Freudenberg, M.; Galanos, C.; Salomão, Reinaldo [UNIFESP] (Wiley-Blackwell, 2002-02-15)
      Background: the use of whole blood (WB) in studying lipopolysaccharide (LPS)-induced cellular activation preserves the milieu in which LPS-cell interaction occurs in vivo. However, little information is available on using ...

    • Isolation and characterization of heparin from tuna skins 

      Jeske, Walter P.; McDonald, Meredith K.; Hoppensteadt, Debra A.; Bau, Elaine C.; Mendes, Aline; Dietrich, Carl P.; Walenga, Jeanine M.; Coyne, Erwin (Sage Publications Inc, 2007-04-01)
      This study characterized heparin isolated from tuna skins. Glycosaminoglycans were isolated from tuna skin after digestion using anion exchange resin. Heparin was eluted from the resin by sodium chloride gradient and was ...