Browsing by Subject "cloning"

Now showing items 1-6 of 6

    • Characterization of Schistosoma mansoni ATPDase2 gene, a novel apyrase family member 

      Levano-Garcia, Julio; Mortara, Renato A.; Verjovski-Almeida, Sergio; DeMarco, Ricardo (Elsevier B.V., 2007-01-12)
      Schistosoma mansoni is a major causative agent of schistosomiasis, which constitutes a severe health problem in developing countries. We have previously described the SmATPDase1 gene, encoding a protein from the external ...

    • Cloning and characterization of a gene encoding a novel immunodominant antigen of Trypanosoma cruzi 

      Lesenechal, M.; Duret, L.; Cano, M. I.; Mortara, R. A.; Jolivet, M.; Camargo, M. E.; daSilveira, J. F.; ParanhosBaccala, G. (Elsevier B.V., 1997-08-01)
      A Trypanosoma cruzi genomic expression library was screened with a pool of sera obtained from chronic chagasic patients. the recombinant antigen (Tc40) isolated from this library reacted with a large number of serum samples ...

    • Cloning and expression of a functionally active truncated N-glycosylated KSHVORF4/KCP/Kaposica in the methylotrophic yeast Pichia pastoris 

      Pereira, NAG; Juliano, M. A.; Carmona, A. K.; Sturrock, E. D.; Kotwal, G. J.; Lahiri, D. K. (New York Acad Sciences, 2005-01-01)
      Kaposi's sarcoma herpesvirus (KSHV) is a typical DNA virus that is associated with a number of proliferative diseases including Kaposi's sarcoma. the KSHV open reading frame (ORF) 4 encodes a complement regulatory protein ...

    • Cloning, Characterization and Anti-Inflammatory Properties of Bothrops jararaca Snake Antithrombin 

      Morais-Zani, Karen de; Grego, Kathleen Fernandes; Torquato, Ricardo José Soares [UNIFESP]; Silva, Caroline Serino; Tanaka, Aparecida Sadae [UNIFESP]; Tanaka-Azevedo, Anita Mitico (Bentham Science Publ Ltd, 2015-01-01)
      Antithrombin inhibits blood coagulation through the interaction with serine proteases in both intrinsic and extrinsic pathways. In addition, antithrombin also shows anti-inflammatory properties, which are independent of ...

    • Cloning, expression and characterization of Bauhinia variegata trypsin inhibitor BvTI 

      Souza, A. F. de; Torquato, RJS; Tanaka, A. S.; Sampaio, CAM (Walter de Gruyter & Co, 2005-11-01)
      A Bauhinia variegata trypsin inhibitor (BvTI) cDNA fragment was cloned into the pCANTAB5E phagemid. the clone pAS 1.1.3 presented a cDNA fragment of 733 bp, including the coding region for a mature BvTI protein comprising ...

    • Molecular characterization and immunolocalization of Schistosoma mansoni ATP-diphosphohydrolase 

      DeMarco, R.; Kowaltowski, A. T.; Mortara, R. A.; Verjovski-Almeida, S. (Elsevier B.V., 2003-08-08)
      Schistosoma mansoni, a human parasite that constitutes a major health problem in developing countries, escapes from host defenses and survives in the human bloodstream. Here, we report the cloning of a S. mansoni ...